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Purified anti-human CD131 Antibody

Pricing & Availability
Clone
1C1 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3R common β chain, CDw131
Isotype
Mouse IgG1, κ
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Product Citations
publications
1C1_Purified_071007
CD131 transfected cell stained with purified 1C1, followed by anti-mouse IgG FITC
  • 1C1_Purified_071007
    CD131 transfected cell stained with purified 1C1, followed by anti-mouse IgG FITC
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306102 100 µg 101 CHF
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Description

CD131, also known as the IL-3R common β subunit (βC), is a 95-120 kD type I transmembrane glycoprotein and belongs to the Ig superfamily. The common β subunit associates with the specific α subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response. The 1C1 antibody is a non-blocking antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3R β chain transfected COS cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2 and immunoprecipitation2. The 1C1 antibody does not block binding of IL-3 and is a non-neutralizing antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Stomski F, et al. 1998. J. Biol. Chem. 273:1192. (WB)
  2. Stomski F, et al. 1999. Blood 94:1933. (IP WB)
  3. Mark A, et al. 2000. Mol. Cell 6:99.
RRID
AB_2229992 (BioLegend Cat. No. 306102)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with the α subunit of IL-3, IL-5, GM-CSF receptors, 95-120 kD
Distribution

Low levels on monocytes, granulocytes, eosinophils, basophils, hematopoietic progenitors, endothelial cells

Function
Signal transducing molecule of IL-3, IL-5, GM-CSF receptors
Ligand/Receptor
IL-3, IL-5, GM-CSF
Cell Type
Basophils, Endothelial cells, Eosinophils, Granulocytes, Hematopoietic stem and progenitors, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sun Q, et al. 1999. Blood 94:1943.
2. Woodcock J, et al. 1997. Blood 90:3005.
3. Lopez A, et al. 1991. J. Biol. Chem. 266:24741.

Gene ID
1439 View all products for this Gene ID
UniProt
View information about CD131 on UniProt.org
Go To Top Version: 2    Revision Date: 08.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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