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Purified anti-human CD137L (4-1BB Ligand) Antibody

Pricing & Availability
Clone
5F4 (See other available formats)
Regulatory Status
RUO
Other Names
4-1BBL, CD137L, TNFSF9, 41BBL
Isotype
Mouse IgG1, κ
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Product Citations
publications
5F4
HuT 78 (T lymphoma cell line) stained with purified 5F4, then detected with biotinylated anti-mouse IgG followed by Sav-PE
  • 5F4
    HuT 78 (T lymphoma cell line) stained with purified 5F4, then detected with biotinylated anti-mouse IgG followed by Sav-PE
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311502 100 µg 129 CHF
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Description

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed
Product Citations
  1. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  2. Ho W, et al. 2013. Cancer Res. 73:652. PubMed
  3. Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed
  4. Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
  5. Vidard L, et al. 2019. J Immunol. 203:676. PubMed
RRID
AB_314881 (BioLegend Cat. No. 311502)

Antigen Details

Structure
TNF superfamily, 97 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T cell proliferation, cytokine production
Ligand/Receptor
4-1BB (CDw137)
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Akiba H, et al. 2000. J. Exp. Med. 191:375. 
2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488. 
3. DeBenedette MA, et al. 1997. J. Immunol. 158:551. 
4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.

Gene ID
8744 View all products for this Gene ID
UniProt
View information about CD137L on UniProt.org
Go To Top Version: 3    Revision Date: 08.23.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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