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Purified anti-human CD172b (SIRPβ) Antibody

Pricing & Availability
Clone
B4B6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
SIRPb, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B4B6_Pure_030308
Human peripheral blood granulocytes stained with purified B4B6, followed by biotinylated anti-mouse IgG and Sav-PE
  • B4B6_Pure_030308
    Human peripheral blood granulocytes stained with purified B4B6, followed by biotinylated anti-mouse IgG and Sav-PE
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323902 100 µg 118 CHF
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Description

The B4B6 antibody recognizes human CD172b also known as signal-regulatory protein beta, and SIRP-beta. CD172b is a member of the signal regulatory protein family and immunoglobulin superfamily. CD172b contains a single pass transmembrane region with immunoglobulin-like domains and unlike CD172a, this receptor lacks a cytoplasmic SHP-2 binding domain. CD172b is a disulfide-linked homodimer with a predicted molecular weight of approximately 43 kD. Two isoforms of CD172b are produced by alternative splicing. CD172b is expressed on myeloid cells including monocytes and dendritic cells where it functions in the negative regulation of tyrosine-kinase coupled signaling processes. CD172b has been shown to regulate neutrophil transepithelial migration. CD172b interacts with DAP12 and Syk kinase (through TYROBP). The B4B6 antibody has been shown to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with SIRPbeta
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Seiffert M, et al. 2001. Blood 97:2741.
Product Citations
  1. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_2301799 (BioLegend Cat. No. 323902)

Antigen Details

Structure
Member of the signal regulatory protein family and immunoglobulin superfamily. Single pass transmembrane receptor with immunoglobulin-like domains; lacks cytoplasmic SHP-2 binding domain. Disulfide-linked homodimer, predicted MW ~43 kD.
Distribution

Myeloid cells, monocytes, dendritic cells

Function
Negative regulation of tyrosine-kinase coupled signaling processes. Regulates neutrophil transepithelial migration
Interaction
DAP12 and Syk (through TYROBP)
Cell Type
Dendritic cells, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kharitonenkov A, et al. 1997. Nature 386:181.
2. Tomasello E, et al. 2000. Eur. J. Immunol. 30:2147.

Gene ID
10326 View all products for this Gene ID
UniProt
View information about CD172b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08.17.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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