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Purified anti-human CD196 (CCR6) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
G034E3 (See other available formats)
Regulatory Status
RUO
Other Names
BN-1, CKR6, DCR2, CKRL3, DRY-6, GPR29, CKR-L3, CMKBR6, GPRCY4, STRL22, GPR-CY4
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
G034E3_Purified_CD196_Cytof_060314.jpg
Human PBMCs stained with 169Tm-anti-CD19 (HIB19) and 141Pr-anti-CD196 (G034E3). CD45+ CD33- cells are displayed in the analysis. Data provided by DVS Sciences.
  • G034E3_Purified_CD196_Cytof_060314.jpg
    Human PBMCs stained with 169Tm-anti-CD19 (HIB19) and 141Pr-anti-CD196 (G034E3). CD45+ CD33- cells are displayed in the analysis. Data provided by DVS Sciences.
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353427 100 µg 188 CHF
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Description

CCR6, also known as CD196, is a chemokine receptor that is expressed on immature dendritic cells, B lymphocytes, and memory T cells. CCR6 binds CCL20, although members of the β defensin family also bind CCR6 with a lower affinity. CCR6 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers Patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both normal and psoriatic skin. CCR6 and/or CCL20 have been implicated in the pathogenesis of rheumatoid arthritis and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR6-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Product Citations
  1. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  2. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  3. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  4. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
RRID
AB_2563725 (BioLegend Cat. No. 353427)

Antigen Details

Structure
Chemokine receptor, GPCR, seven transmembrane receptor
Distribution

Immature dendritic cells, B lymphocytes, memory T cells, Th17 cells

Function
Host defense, important for recruitment of B cells to secondary lymphoid organs, mediates arrest of T cells on dermal endothelial cells
Ligand/Receptor
CCL20 (MIP-3α)
Cell Type
B cells, Dendritic cells, T cells, Th17
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1996. Biochem. Bioph. Res. Co. 227:846.
2. Yang D, et al. 1999. Science 286:525.
3. MacDonald KG, et al. 2007. Am. J. Pathol. 170:1229.
4. Homey B, et al. 2000. J. Immunol. 164:6621.
5. Hirota K, et al. 2007. J. Exp. Med. 204:2803.
6. Singh SP, et al. 2008. J. Immunol. 180:214.

Gene ID
1235 View all products for this Gene ID
UniProt
View information about CD196 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD196 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD196 (CCR6) G034E3 FC
Alexa Fluor® 647 anti-human CD196 (CCR6) G034E3 FC
Brilliant Violet 421™ anti-human CD196 (CCR6) G034E3 FC
PerCP/Cyanine5.5 anti-human CD196 (CCR6) G034E3 FC
PE anti-human CD196 (CCR6) G034E3 FC
FITC anti-human CD196 (CCR6) G034E3 FC
Alexa Fluor® 488 anti-human CD196 (CCR6) G034E3 FC
APC anti-human CD196 (CCR6) G034E3 FC
PE/Cyanine7 anti-human CD196 (CCR6) G034E3 FC
Brilliant Violet 605™ anti-human CD196 (CCR6) G034E3 FC
Brilliant Violet 785™ anti-human CD196 (CCR6) G034E3 FC
Brilliant Violet 510™ anti-human CD196 (CCR6) G034E3 FC
Brilliant Violet 650™ anti-human CD196 (CCR6) G034E3 FC
Purified anti-human CD196 (CCR6) (Maxpar® Ready) G034E3 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD196 (CCR6) G034E3 FC
APC/Cyanine7 anti-human CD196 (CCR6) G034E3 FC
Alexa Fluor® 700 anti-human CD196 (CCR6) G034E3 FC
Brilliant Violet 711™ anti-human CD196 (CCR6) G034E3 FC
TotalSeq™-A0143 anti-human CD196 (CCR6) G034E3 PG
Pacific Blue™ anti-human CD196 (CCR6) G034E3 FC
TotalSeq™-C0143 anti-human CD196 (CCR6) G034E3 PG
APC/Fire™ 750 anti-human CD196 (CCR6) G034E3 FC
TotalSeq™-B0143 anti-human CD196 (CCR6) G034E3 PG
TotalSeq™-D0143 anti-human CD196 (CCR6) G034E3 PG
PE/Fire™ 640 anti-human CD196 (CCR6) G034E3 FC
APC/Fire™ 810 anti-human CD196 (CCR6) G034E3 FC
Spark NIR™ 685 anti-human CD196 (CCR6) G034E3 FC
PE/Fire™ 700 anti-human CD196 (CCR6) G034E3 FC
PE/Cyanine5 anti-human CD196 (CCR6) G034E3 FC
Biotin anti-human CD196 (CCR6) G034E3 FC
PE/Fire™ 810 anti-human CD196 (CCR6) G034E3 FC
Go To Top Version: 2    Revision Date: 07.22.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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