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Purified anti-human CD20 Antibody

Pricing & Availability
Clone
C20Mab-60 (See other available formats)
Regulatory Status
RUO
Other Names
B1, Bp35
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
C20Mab-60_Purified_CD20_Antibody_033122
Human peripheral blood lymphocytes were surface stained with anti-human CD19 APC and purified anti-human CD20 (clone C20Mab-60) (left), or mouse IgG2a, κ isotype control (right) followed by PE goat anti-mouse IgG.
  • C20Mab-60_Purified_CD20_Antibody_033122
    Human peripheral blood lymphocytes were surface stained with anti-human CD19 APC and purified anti-human CD20 (clone C20Mab-60) (left), or mouse IgG2a, κ isotype control (right) followed by PE goat anti-mouse IgG.
  • C20Mab-60_Purified_CD20_Antibody_2_033122
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with 1X Tris-EDTA buffer (pH 9.0) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD20 (clone C20Mab-60) and 5 µg/mL of purified anti-human CD4 (clone A17070D) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 647 anti-mouse IgG2a (red) and Alexa Fluor® 594 anti-mouse IgG2b (green) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • C20Mab-60_Purified_CD20_Antibody_3_033122
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with 1X Tris-EDTA buffer (pH 9.0) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD20 (clone C20Mab-60) and 5 µg/mL of purified anti-human CD4 (clone A17070D) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 647 anti-mouse IgG2a (red) and Alexa Fluor® 594 anti-mouse IgG2b (green) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • C20Mab-60_Purified_CD20_Antibody_4_033122
    Human paraffin-embedded B cell lymphoma tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with 1X Tris-EDTA buffer (pH 9.0) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD20 (clone C20Mab-60) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-mouse IgG (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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382802 100 µg 77 CHF
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LN229/CD20 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified 
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2924581 (BioLegend Cat. No. 382802)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Furusawa Y, et al. 2020. Monoclon Antib Immunodiagn Immunother. 39:112-116.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org
Go To Top Version: 1    Revision Date: 03.31.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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