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Purified anti-human CD221 (IGF-1R) Antibody

Pricing & Availability
Clone
1H7/CD221 (See other available formats)
Regulatory Status
RUO
Other Names
Insulin-like growth factor 1 receptor (IGF1R)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1H7slashCD221_Pure_042211
Human peripheral blood lymphocytes were stained with purified CD221 (clone 1H7/CD221) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
  • 1H7slashCD221_Pure_042211
    Human peripheral blood lymphocytes were stained with purified CD221 (clone 1H7/CD221) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
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351802 100 µg 141 CHF
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Description

CD221, also known as insulin-like growth factor 1 receptor (IGF1R), is a 135 kD transmembrane receptor. It is widely expressed on lymphoid and myeloid cells as well as non-haematopoietic cells. CD221 is a tyrosine kinase receptor and the ligands for CD221 are insulin-like growth factor 1 and 2 (IGF-1 and IGF-2), the polypeptide hormones similar in molecular structure to insulin. IGF-1/2 binding activates downstream PI3K and MAPK pathways and plays an important role in cell growth. Recent studies reveal its function of recruiting immune cells during tissue repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
IGF-1 receptor purified from human placentas
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been published as 1H7. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining of frozen tissue sections2, and blocking functions2-5.

This product is offered for sale and sold pursuant to a license to seller by City of Hope and may be used only as a research reagent.  Use for any other purpose is expressly not authorized.

Application References

(PubMed link indicates BioLegend citation)
  1. Li SL, et al. 1993. Biochem. Bioph. Res. Co. 196:92. (WB IP)
  2. Li SL, et al. 2000. Cancer Immunol. Immun. 49:243. (IHC Block)
  3. Ye JJ, et al. 2003. Horm. Metab. Res. 35:836. (Block)
  4. Kusada Y, et al. 2008. J. Biochem. 143:9. (Block)
  5. Ohtani M, et al. 2009. Biosci. Trends. 3:131. (Block)
RRID
AB_10896950 (BioLegend Cat. No. 351802)

Antigen Details

Structure
IGF-1R contains 2 α subunits and 2 β subunits, 135 kD
Distribution

Haematopoietic cells and non-haematopoietic cells

Function
Cell growth and immune cells recruitment
Ligand/Receptor
IGF-1, IGF-2
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Baudler S, et al. 2005. J. Immunol. 174:5516.
2. Sharp LL, et al. 2005. Nat. Immunol. 6:73.

Gene ID
3480 View all products for this Gene ID
UniProt
View information about CD221 on UniProt.org
Go To Top Version: 2    Revision Date: 08.16.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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