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Purified anti-human CD248 (Endosialin) Antibody

Pricing & Availability
Clone
B1/35 (See other available formats)
Regulatory Status
RUO
Other Names
CD248 molecule, Tumor endothelial marker 1(TEM1), CD164 sialomucin-like 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
B1-35_PURE_CD248_Antibody_121322
Human LWB were stained with anti-human CD8 APC and purified anti-human CD248 (Endosialin) (clone B1/35) (left) or with purified mouse IgG1, κ isotype control (right) followed by PE anti-mouse IgG1.
  • B1-35_PURE_CD248_Antibody_121322
    Human LWB were stained with anti-human CD8 APC and purified anti-human CD248 (Endosialin) (clone B1/35) (left) or with purified mouse IgG1, κ isotype control (right) followed by PE anti-mouse IgG1.
  • B1-35_PURE_CD248_Antibody_2_110722
    Human bone marrow-derived mesenchymal stem cells were stained with purified anti-human Endosialin (clone B1/35) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
  • B1-35_PURE_CD248_Antibody_3a_121322
    Human paraffin-embedded breast tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X (10 mM, pH 6.0) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/mL purified anti-human CD248 (Endosialin) (clone B1/35) and 5 µg/mL purified Keratin 14 polyclonal (clone Poly19053) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 647 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor® 594 donkey anti-rabbit IgG (clone Poly4064) (green) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 20X objective.
  • B1-35_PURE_CD248_Antibody_4a_121322
    Human bone marrow-derived mesenchymal stem cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with 5 µg/mL purified anti-human CD248 (Endosialin) (clone B1/35) in blocking buffer overnight at 4°C. On the next day, the cells were washed twice with PBS and stained with 2.5 µg/mL Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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379102 100 µg 335 CHF
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Description

Endosialin, also known as Tumor Endothelial Marker 1 (TEM1), CD248 and CD164 sialomucin-like 1 (CD164L1), is classified as a C-type lectin-like protein and shares both sequence and structural 39% homology with thrombomodulin (CD141) and 33% homology to complement receptor C1qRp (CD93). CD248 is expressed on pericytes and fibroblasts during tissue development, tumor neovascularization and inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Diploid human fibroblasts
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)

1. Teicher BA. 2019. Oncotarget. 10:993-1009. (Flow, ICC)
2. Hardie DL, et al. 2011. Immunology. 133:288-95. (IHC-P, Flow)

RRID
AB_2927975 (BioLegend Cat. No. 379102)

Antigen Details

Structure
Heavily glycosylated, single-pass transmembrane protein
Distribution

Endosialin is expressed on pericytes and fibroblasts during tissue development, tumor neovascularization and inflammation.

Cell Type
B cells, Dendritic cells, Fibroblasts, Mesenchymal Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Bagley RG, et al. 2009. Int J Oncol. 34:619-27. 
  2. Teicher BA. 2019. Oncotarget. 10:993-1009.
  3. Teicher BA. 2007. Int J Oncol. 2007. 30:305-12.
  4. Rouleau C, et al. 2008. Clin Cancer Res. 14:7223-36.
Gene ID
57124 View all products for this Gene ID
UniProt
View information about CD248 on UniProt.org
Go To Top Version: 1    Revision Date: 11.07.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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