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Purified anti-human CD26 Antibody

Pricing & Availability
Clone
5-109 (See other available formats)
Regulatory Status
RUO
Other Names
ADA-binding protein, DPP IV ectoenzyme, EC 3.4.14.5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. 5-109_PURE_CD26_FC_072924
Human peripheral blood lymphocytes were stained with purified anti-human CD26 (clone 5-109) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then stained with anti-human CD3 (clone UCHT1) APC.
  • A. 5-109_PURE_CD26_FC_072924
    Human peripheral blood lymphocytes were stained with purified anti-human CD26 (clone 5-109) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG PE. Cells were then stained with anti-human CD3 (clone UCHT1) APC.
  • B. 5-109_PURE_CD26_Direct-ELISA_072924
    Direct ELISA showing purified anti-human CD26 (clone 5-109) bound to plate-immobilized recombinant human CD26 (DPP4) (Cat. No. 764104). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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335306 100 µg 101 CHF
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Description

CD26, also known as ADA-binding protein and dipeptidyl peptidase IV (DPPIV), is a 110 kD type II membrane protein. It is a member of the peptidase and ectoenzyme family. CD26 is expressed on the membrane of mature thymocytes, T lymphocytes (upregulated upon activation), B cells, NK cells, and macrophages. CD26 cleaves off N-terminal X-Pro and X-Ala dipeptides from polypeptides. It plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45, and ADA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Direct ELISA – Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. Each lot of this antibody is verified by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Application Notes

Test clone 5-109 and reference clone BA5b completely cross-block the staining on target cells.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone 5-109) could function as a capture antibody when paired with biotin anti-human CD26 (DDP4) (Cat No. 628153) (clone 1-10) as the detection antibody and using recombinant Human DDP4 (carrier-free) protein (Cat. Nos. 764102, 764104, and 764106) as the assay standard.

RRID
AB_3662413 (BioLegend Cat. No. 335306)

Antigen Details

Structure
Peptidases and ectoenzyme families, type II glycoprotein, 110 kD
Distribution

Thymocyte subset, memory T cells, B cells, NK cells, epithelial cells, macrophages

Function
Dipeptidyl peptidase, T cell costimulation, HIV entry
Ligand/Receptor
Adenosine-deaminase, collagen
Cell Type
B cells, Epithelial cells, Macrophages, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Kameoka J, et al. 1993. Science. 261:466-9.
  2. Dang NH, et al. 1990. J Exp Med. 172:649-52.
Gene ID
1803 View all products for this Gene ID
UniProt
View information about CD26 on UniProt.org
Go To Top Version: 1    Revision Date: 07.16.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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