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Purified anti-human CD26 (DDP4) Antibody

Pricing & Availability
Clone
1-10 (See other available formats)
Regulatory Status
RUO
Other Names
CD26, Adenosine deaminase binding protein (ADABP), Adenosine deaminase complexing protein2 (ADCP-2), Dipeptidyl peptidase IV (DPP IV)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1-10_PURE_CD26_Direct-ELISA_100424
Direct ELISA showing purified anti-human CD26 (DDP4) (clone 1-10) bound to plate-immobilized recombinant human DDP4 (Cat. Nos. 764102, 764104, and 764106). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • 1-10_PURE_CD26_Direct-ELISA_100424
    Direct ELISA showing purified anti-human CD26 (DDP4) (clone 1-10) bound to plate-immobilized recombinant human DDP4 (Cat. Nos. 764102, 764104, and 764106). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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628156 100 µg 445 CHF
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Description

Dipeptidyl peptidase IV (DPP4) is a multifunctional type II transmembrane serine peptidase. It is also known as a cell surface glycoprotein receptor, CD26, which is involved in the co-stimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. It acts as a positive regulator of T-cell co-activation, by binding ADA, CAV1, IGF2R, and PTPRC at the very least. DPP4 is widely expressed in many specialized cell types and has distinct functions independently of its form, anchored to the membrane or in soluble form. The soluble form of DPP4 appears to be derived primarily from endothelial cells, epithelial cells, and leukocytes. It is upregulated in proinflammatory states including obesity, type-2 diabetes mellitus, and atherosclerosis. DPP4 is a serine aminopeptidase that cleaves preferentially Xaa–Pro dipeptides from oligopeptides with a typical length of 30 amino acids, but tolerates other small residues at P1-position. Its biological substrates include many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Thus, it regulates various physiological processes by cleaving peptides in the circulation on the cell surface. It has been discovered that DPP4 may be a receptor for receptor binding domain (RBD) of spike entry glycoprotein in Middle East respiratory syndrome coronavirus (MERS-CoV).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant DDP4 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone 1-10) could function as a detection antibody when paired with purified anti-human CD26 (DDP4) (Cat No. 335306) (clone 5-109) as the capture antibody and using Recombinant Human DDP4 (carrier-free) protein (Cat. Nos. 764102, 764104, and 764106) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

Antigen Details

Distribution

High expression in RPTEC/TERT1, BJ-hTERT, and ASC-TERT1 cell line; moderate expression in PC-3 and Hep G2 cell line

Interaction
T-Cell, lymphocyte
Ligand/Receptor
Adenosin deaminase (ADA), RANTES, stromal cell-derived factor (SDF)-1α, glucose-dependent insulinotropic polypeptide (GIP), and glucagon-like peptide-1 (GLP-1)
Cell Type
Embryonic Stem Cells
Biology Area
Angiogenesis, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Metzemaekers M, et al. 2016. Front in Immunology. 7:483.
  2. Song W, et al. 2014. Virology. 473:49-53.
  3. Ohnuma K, et al. 2007. J Biol Chem. 282:10117-10131.
  4. Engel M, et al. 2003. PNAS USA. 100:5063.
  5. Morimoto C, et al. 1989. J Immunol. 143:3430-9.
Gene ID
1803 View all products for this Gene ID
UniProt
View information about CD26 on UniProt.org
Go To Top Version: 1    Revision Date: 10.04.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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