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Purified anti-human CD266 Antibody

Pricing & Availability
Clone
A19192A (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast growth factor-inducible 14 kD protein (Fn14), TWEAK receptor, TNFRSF12A
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A19192A_PURE_CD266_Antibody_122221
Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD266 (clone A19192A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Goat anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • A19192A_PURE_CD266_Antibody_122221
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD266 (clone A19192A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Goat anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • A19192A_PURE_CD266_Antibody_2_122221
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD266 (clone A19192A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Goat anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
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379302 100 µg 229 CHF
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Description

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910445 (BioLegend Cat. No. 379302)

Antigen Details

Structure
Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
Distribution

Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC

Function
Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
Ligand/Receptor
TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
Antigen References
  1. Feng SL, et al. 2000. Am J Pathanol. 156:1253-61.
  2. Meighan-Mantha RL, et al. 1999. J Biol Chem. 274:33166-79.
  3. Nakayama M, et al. 2003. J Immunol. 170:341-8.
  4. Lynch CN, et al. 1999. J Biol Chem. 274:8455-9.

 

Gene ID
51330 View all products for this Gene ID
UniProt
View information about CD266 on UniProt.org
Go To Top Version: 1    Revision Date: 12.22.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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