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Purified anti-human CD281 (TLR1) Antibody

Pricing & Availability
Clone
TLR1.136 (See other available formats)
Regulatory Status
RUO
Other Names
CD281, TLR1, TLR-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TLR1dot136_Pure_082808.jpg
Human peripheral blood monocytes stained with purified TLR1.136, followed by biotinylated anti-mouse IgG and Sav-PE
  • TLR1dot136_Pure_082808.jpg
    Human peripheral blood monocytes stained with purified TLR1.136, followed by biotinylated anti-mouse IgG and Sav-PE
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334502 100 µg 258 CHF
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Description

Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been indentified. Toll-like receptor 1 (TLR1), known as CD281, is expressed on monocytes/macrophages, dendritic cells, and granulocytes and some lymphocytes at low levels. By association with TLR2, TLR1 is a coreceptor involved in the response to diverse set of pathogens, such as triacylated lipopeptidess (but not diacylated lipopeptides), peptidoglycans, lipoteichoic acid.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR1 transfected Ba/F3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Other reported applications include immunoprecipitation1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakao Y, et al. 2005. J. Immunol. 174:1566. (BA IP FC IF) PubMed
RRID
AB_1186023 (BioLegend Cat. No. 334502)

Antigen Details

Structure
Type I transmembrane protein characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain, heterodimer with TLR2
Distribution

Monocytes/macrophages, dendritic cells

Function
Coreceptor with TLR2 to activate innate host defense against diverse set of pathogens
Ligand/Receptor
Microbial lipoproteins, peptidoglycans, lipoteichoic acid
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Hoebe K, et al. 2005. Nature 433:523.
2. Sandor F, et al. 2003. J. Cell Biol. 162:1099.
3. Takeuchi O, et al. 2002. J. Immunol. 169:10.
4. Wyllie D, et al. 2000. J. Immunol. 165:7125.
5. Buwitt-Beckmann U, et al. 2006. J. Biol. Chem. 281:9049.

Gene ID
7096 View all products for this Gene ID
UniProt
View information about CD281 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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