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Purified anti-human CD309 (VEGFR2) Antibody

Pricing & Availability
Clone
7D4-6 (See other available formats)
Regulatory Status
RUO
Other Names
KDR, Vascular Endothelial Receptor 2 (VEGF-R2, VEGFR2, VEGFR)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a)7D4-6_Purified_VEGFR1_Antibody_FC_091013.jpg
HUVEC human endothelial cells were stained with purified CD309 (clone 7D4-6) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • a)7D4-6_Purified_VEGFR1_Antibody_FC_091013.jpg
    HUVEC human endothelial cells were stained with purified CD309 (clone 7D4-6) (filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • b)7D4-6_Purified_CD309_Antibody_IF_022514
    HUVEC cells were fixed with 1% paraformaldehyde (PFA) and then stained with 10 µg/ml of purified CD309 (VEGFR2) (clone 7D4-6) at 4°C overnight, followed by DyLight™ 594 anti-mouse IgG staining at 4°C for 2 hours (red). Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
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359902 100 µg 229 CHF
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Description

CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human KDR recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Fujiwara K, et al. 2020. Cancers (Basel). 12:. PubMed
RRID
AB_2562485 (BioLegend Cat. No. 359902)

Antigen Details

Structure
Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family
Distribution

Endothelial cells, embryonic tissues

Ligand/Receptor
VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.
2. Ferrara N and Gerber HP. 2002. Acta. Haematol. 106:148.
3. Murohara T, et al. 1998. Circulation. 97:99.

Gene ID
3791 View all products for this Gene ID
UniProt
View information about CD309 on UniProt.org
Go To Top Version: 4    Revision Date: 06.02.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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