Purified anti-human CD326 EpCAM Maxpar Ready Antibody anti-CD326

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Clone: 9C4 (See other available formats)
Regulatory Status: RUO
Other Names: Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype: Mouse IgG2b, κ
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Product Citations: publications

Human HT-29 colon carcinoma cells (top) and human Jurkat T cells (bottom) stained with 141Pr-anti-CD326 (9C4). Data provided by DVS Sciences.

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324229

100 µg

229 CHF

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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: DU.4475 breast carcinoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry³, and spatial biology (IBEX)^4,5.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563742 (BioLegend Cat. No. 324229)

Antigen Details

Structure: Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution: Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
Function: Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification: Glycosylated.
Cell Type: Embryonic Stem Cells, Epithelial cells
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.
Gene ID: 4072 View all products for this Gene ID
UniProt: View information about CD326 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD326 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD326 (EpCAM)	9C4	FC,ICC,IHC
FITC anti-human CD326 (EpCAM)	9C4	FC
PE anti-human CD326 (EpCAM)	9C4	FC
APC anti-human CD326 (EpCAM)	9C4	FC
Alexa Fluor® 488 anti-human CD326 (EpCAM)	9C4	FC,SB
Alexa Fluor® 647 anti-human CD326 (EpCAM)	9C4	FC,ICC,SB
PerCP/Cyanine5.5 anti-human CD326 (EpCAM)	9C4	FC,SB
Biotin anti-human CD326 (EpCAM)	9C4	FC
Pacific Blue™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 421™ anti-human CD326 (EpCAM)	9C4	FC
PE/Cyanine7 anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 605™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 650™ anti-human CD326 (EpCAM)	9C4	FC
Alexa Fluor® 594 anti-human CD326 (EpCAM)	9C4	IHC-P,ICC,SB
Purified anti-human CD326 (EpCAM) (Maxpar® Ready)	9C4	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD326 (EpCAM)	9C4	FC
APC/Fire™ 750 anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 510™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 785™ anti-human CD326 (EpCAM)	9C4	FC
Brilliant Violet 711™ anti-human CD326 (Ep-CAM)	9C4	FC
TotalSeq™-A0123 anti-human CD326 (Ep-CAM)	9C4	PG
APC/Cyanine7 anti-human CD326 (Ep-CAM)	9C4	FC
Alexa Fluor® 700 anti-human CD326 (EpCAM)	9C4	FC
TotalSeq™-C0123 anti-human CD326 (Ep-CAM)	9C4	PG
TotalSeq™-B0123 anti-human CD326 (Ep-CAM)	9C4	PG
PE/Cyanine5 anti-human CD326 (Ep-CAM)	9C4	FC
TotalSeq™-D0123 anti-human CD326 (Ep-CAM)	9C4	PG
Spark UV™ 387 anti-human CD326 (Ep-CAM)	9C4	FC
Spark Red™ 718 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC
Spark Blue™ 574 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC
Spark Blue™ 550 anti-human CD326 (Ep-CAM) (Flexi-Fluor™)	9C4	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07.22.2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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