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Purified anti-human CD340 (erbB2/HER-2) Antibody

Pricing & Availability
Clone
H2Mab-77 (See other available formats)
Regulatory Status
RUO
Other Names
HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, oncogene NGL, neuroblastoma- or glioblastoma-derived, HER-2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H2Mab-77_PURE_CD340_Antibody_1_012621.png
Human paraffin-embedded HER2 positive patient breast cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD340 (erbB2/HER-2) (clone H2Mab-77) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (Poly4053) antibody (red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • H2Mab-77_PURE_CD340_Antibody_1_012621.png
    Human paraffin-embedded HER2 positive patient breast cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD340 (erbB2/HER-2) (clone H2Mab-77) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (Poly4053) antibody (red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • H2Mab-77_PURE_CD340_Antibody_2_012621.png
    Human paraffin-embedded normal breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citric buffer 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL purified anti-human CD340 (erbB2/HER-2) (clone H2Mab-77) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (Poly4053) antibody (red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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399402 100 µg 335 CHF
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Description

CD340 is also known as HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, and oncogene NGL, neuroblastoma- or glioblastoma-derived. It is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain and has an approximate molecular weight of 185 kD. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region (ECR). The HER2/erbB2 protein is expressed on many tumor cells, including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow mesenchymal stem cells. Although the erbB2/HER2 protein contains no ligand binding domain, this protein interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers. The erbB2/HER-2 protein has been shown to interact with a large number of proteins, including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others. CD340 is extensively modified by tyrosine phosphoryation on multiple residues (Y1023, Y1139, Y1196, Y1221, Y1222, Y1248).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HER2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890848 (BioLegend Cat. No. 399402)

Antigen Details

Structure
Member of the epidermal growth factor receptor family of cell membrane tyrosine kinases. Single transmembrane pass protein with an approximate molecular weight of 185 kD. Contains three furin repeats and two cheY homologous receiver domains in the ECR.
Distribution

Expressed on many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow MSCs.

Function
Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
Interaction
CD340 (HER2/erbB2) interacts with a large number of proteins, including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others.
Antigen References

1. Akiyama T, et al. 1986. Science 232:1644.
2. Bargmann CI, et al. 1986. Nature 319:226.
3. Pegram MD, et al. 1997. Oncogene 15:537.
4. Slamon DJ, et al. 1989. Science 244:707.
5. Leone F, et al. 2003. J. Leukocyte Biol. 74:593.

Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01.26.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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