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Purified anti-human CD35 Antibody

Pricing & Availability
Clone
E11 (See other available formats)
Regulatory Status
RUO
Workshop
III 204
Other Names
C3b/C4b receptor, complement receptor type 1 (CR1)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
E11_Pure_060308
Human peripheral blood lymphocytes stained purified E11, followed by anti-mouse IgG FITC
  • E11_Pure_060308
    Human peripheral blood lymphocytes stained purified E11, followed by anti-mouse IgG FITC
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333402 100 µg 114 CHF
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Description

CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Hogg N, et al. 1984. Eur. J. Immunol. 14:236
  2. Zabeleta J, et al. 1998. Clin Diag. Lab. Immunol. 5:690
  3. Pascual M, et. al. 1994. Eur. J. Immunol. 24:702
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Weinhage T, et al. 2020. J Immunol. 205:56. PubMed
  2. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  3. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
RRID
AB_1089032 (BioLegend Cat. No. 333402)

Antigen Details

Structure
Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD
Distribution

Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells

Function
Adhesion, mediate phagocytosis, inhibit T-cell proliferation
Ligand/Receptor
C3b, iC3, C4b, iC4
Cell Type
B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699

Gene ID
1378 View all products for this Gene ID
UniProt
View information about CD35 on UniProt.org
Go To Top Version: 2    Revision Date: 08.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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