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Purified anti-human CD39 Antibody

Pricing & Availability
Clone
S23003F (See other available formats)
Regulatory Status
RUO
Other Names
ENTPD1, ATPDase, NTPDase-1, SPG64, ectonucleoside triphosphate diphosphohydrolase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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A. S23003F_PURE_CD39_FC_1_122024
Human peripheral blood mononuclear cells were stained with purified anti-human CD39 (clone S23003F) (left) or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then washed and stained with anti-human CD4 (clone SK3) APC/Fire™ 750 and anti-human CD3 (clone SK7) APC. Data shown were gated on CD3-positive lymphocytes.
  • A. S23003F_PURE_CD39_FC_1_122024
    Human peripheral blood mononuclear cells were stained with purified anti-human CD39 (clone S23003F) (left) or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then washed and stained with anti-human CD4 (clone SK3) APC/Fire™ 750 and anti-human CD3 (clone SK7) APC. Data shown were gated on CD3-positive lymphocytes.
  • b. S23003F_PURE_CD39_FC_2_122024
    Human peripheral blood mononuclear cells were stained with purified anti-human CD39 (clone S23003F) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE. Cells were then washed and stained with anti-human CD4 (clone SK3) APC/Fire™ 750, anti-human CD127 (clone A019D5) PE/Dazzle™ 594, and anti-human CD25 (clone BC96) PE/Cyanine5. Cells were gated on CD4+CD25+ CD127low regulatory T cells.
  • C. S23003F_Purified_CD39_FC_3_012325
    Human peripheral blood mononuclear cells were stimulated with immobilized Ultra-LEAF™ purified CD3 (clone UCHT1) and Ultra-LEAF™ purified CD28 (clone CD28.2) for 7 days. Cells were then stained with purified anti-human CD39 (clone S23003F) (left) or purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG Alexa Fluor® 488. Cells were washed and stained with anti-human CD366 (Tim-3) (clone A18087E) PE and Helix NP™ Blue (Cat. No. 425305). Data shown was gated on live cells.
  • D. S23003F_PURE_CD39_ICC_4_122024
    CD4+ human T cells were isolated from human peripheral blood mononuclear cells using MojoSort™ Human CD4 Nanobeads (Cat. No. 480013) and spun onto microscope slides. After fixing with Fixation Buffer (Cat. No. 420801) for 30 minutes, the cells were blocked with PBS containing 3% BSA and washed twice. Cells were then stained with 1.25 µg/mL of purified anti-human CD39 (clone S23003F) (panel A) or purified mouse IgG1, κ isotype control (panel B), followed by goat anti-mouse IgG Alexa Fluor® 555 (red). Nuclei were counterstained with DAPI (blue). Scale bar: 10 µm
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315107 100 µg 102 CHF
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Description

Human CD39 is an integral membrane protein with two transmembrane domains. It exists as a homotetramer. Expression of CD39 is found on activated lymphocytes, a subset of T cells and B cells, and dendritic cells with weak staining on monocytes and granulocytes. CD39 and CD73 have been found on regulatory T cells, specifically the effector/memory-like. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. CD39 is the dominant ectonucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD39 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Multi-pass membrane protein
Distribution

Plasma membrane, Caveola

Function
ATP hydrolysis, ATP binding, Hydrolase
Interaction
Activated lymphocytes
Cell Type
B cells, Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Maliszewski CR, et al. 1994. J Immunol. 153:3574-3583.
  2. Kaczmarek E, et al. 1996. J Biol Chem. 271:33116-33122.
  3. Jiang X, et al. 2023. Front Immunol. 14:1110325-1110325.
  4. Timperi E, et al. 2021. Int J Mol Sci. 22:8068-8068.
  5. Mitra A, et al. 2023. Clin Cancer Res. 29:4242-4255.
Gene ID
953 View all products for this Gene ID
UniProt
View information about CD39 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.03.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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