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Purified anti-human CD44 variant 9 (CD44v9) Antibody

Pricing & Availability
Clone
RV3 (See other available formats)
Regulatory Status
RUO
Other Names
CD44 variant 9, CD44 variant isoform 9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RV3_PURE_CD44v9_Antibody_061118
Human prostatic carcinoma cell line, PC3 was stained with purified CD44 isoform 9 (clone RV3, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • RV3_PURE_CD44v9_Antibody_061118
    Human prostatic carcinoma cell line, PC3 was stained with purified CD44 isoform 9 (clone RV3, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • RV3_PURE_CD44v9_Antibody_IHC-P_061118
    Human paraffin-embedded colon cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD44v9 (clone RV3) purified at 4°C overnight, followed by 2.5 µg/ml of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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394402 100 µg 375 CHF
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Description

CD44 isoform 9, also known as CD44v9, is a CD44 isofom consisting of exons 1-5, 13 (v9) and 15 to 19, product of alternative splicing. CD44v9 is expressed by cancer stem cells, epithelial-type carcinomas, subsets of monocytes and subsets of lymphocytes; have a role in tumor initiation, maintenance and metastasis of cancer cells. CD44v9 interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells, and is a potential predictive marker for recurrence of some cancers and a potential therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD44v8-10 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kakehashi, A., et al. 2016. Cancer Sci. 107: 609. (IHC-P)
  2. Aso T, et al. 2015. PLoS One. 10: e0116596. (IHC-P)
  3. Yoshikawa M, et al. 2013. Cancer Res. 73: 1855. (IHC-F)
  4. Ishimoto T, et al. 2011. Cancer Cell. 387-400. (IHC-P)
RRID
AB_2734487 (BioLegend Cat. No. 394402)

Antigen Details

Distribution

Cancer stem cells, epithelial-type carcinomas, subset of monocytes, subset of lymphocytes.

Function
Role in tumor initiation and maintenance and metastasis of cancer cells, Interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells.
Cell Type
Epithelial cells, Lymphocytes, Monocytes, Neutrophils
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Hagiwara M, et al. 2018. BMC Cancer. 18:113
  2. Matsumoto T, et al. 2017. Oncogenesis. 6:397
  3. Bertaux-Skeirik N, et al. 2017. J. Pathol. 242:463
  4. Kodama H, et al. 2017. Br. J. Cancer. 116:186
Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44v9 on UniProt.org
Go To Top Version: 2    Revision Date: 02.22.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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