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Purified anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI30_Purified_071307
Human peripheral blood lymphocytes were stained with purified CD45 (clone HI30) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • HI30_Purified_071307
    Human peripheral blood lymphocytes were stained with purified CD45 (clone HI30) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • HI30_Purified_2_CD45_IHC_071315
    Preparation and staining of formalin fixed paraffin-embedded (FFPE) human tonsil was performed. After antigen retrieval, the sample was incubated with the purified monoclonal antibody (clone HI30) at 0.5 µg/mL for 20 minutes. The Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) was used according to protocol for chromogenic detection.
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304001 25 µg 24 CHF
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304002 100 µg 50 CHF
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-P - Verified
WB, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.1 - 10 µg/ml is suggested, if the antibody is not available in a pre-diluted format. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  3. Nagasawa Y, et al. 2021. PLoS One. 16:e0249340. PubMed
  4. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  5. Lee SA, et al. 2020. J Immunol. 204:586. PubMed
  6. Siris S, et al. 2020. Methods Mol Biol. 2183:9. PubMed
  7. Mishra P, et al. 2021. Indian J Hematol Blood Transfus. 37:391. PubMed
  8. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  9. Canela VH, et al. 2022. Anat Rec (Hoboken). 305:1701. PubMed
  10. Saeki N, et al. 2022. J Clin Invest. 132: . PubMed
  11. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  12. Bordoloi D, et al. 2022. JCI Insight. 7: . PubMed
  13. de Vargas Roditi L, et al. 2022. Cell Rep Med. 3:100604. PubMed
  14. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  15. Chen W, et al. 2023. JCI Insight. 8:. PubMed
  16. Wu T, et al. 2023. Nat Methods. 20:723. PubMed
  17. Mei ZF, et al. 2023. Technol Cancer Res Treat. 22:15330338231167827. PubMed
  18. Pearce DR, et al. 2023. Front Oncol. 13:1156743. PubMed
  19. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  20. Miyamoto DT, et al. 2018. Cancer Discov. 0.533333333. PubMed
  21. Sugimoto H, et al. 2021. AAPS J. 23:36. PubMed
  22. Behrens NE, et al. 2019. MBio. 10:2. PubMed
  23. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  24. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  25. Kuwada K, et al. 2018. J Exp Clin Cancer Res. 37:307. PubMed
  26. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  27. Korn MA, et al. 2020. J Immunol. 205:2595. PubMed
  28. Bokun V, et al. 2019. PLoS One. 14:e0225767. PubMed
  29. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  30. Zhong M, et al. 2021. J Pers Med. 11:. PubMed
  31. Gao H, et al. 2021. J Virol. :. PubMed
  32. Bhat N, et al. 2015. J Immunol. 195: 5178 - 5188. PubMed
  33. Pir GJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  34. Lu-Culligan A, et al. 2021. Med. 2(5):591-610.e10. PubMed
  35. Chow SH, et al. 2020. J Leukoc Biol. 108:967. PubMed
  36. Aspegren O, et al. 2022. Front Neurol. 13:846293. PubMed
  37. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  38. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  39. Arakelyan A, et al. 2017. Sci Rep. 1.46875. PubMed
  40. Gunawan M, et al. 2017. Sci Rep. . 10.1038/s41598-017-16999-7. PubMed
  41. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  42. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  43. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  44. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  45. Elst J, et al. 2022. Front Immunol. 13:835618. PubMed
  46. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  47. Nagano M, et al. 2007. Blood . 110:151. PubMed
  48. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  49. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  50. Bottoni G, et al. 2019. Nat Commun. 10:3884. PubMed
  51. Böttcher C, et al. 2019. Sci Rep. 9:19471. PubMed
  52. Frobel J, et al. 2018. Clin Epigenetics. 10:67. PubMed
  53. Zheng Y, et al. 2022. iScience. 25:103785. PubMed
  54. Woloshuk A, et al. 2021. Cytometry A. 99:707. PubMed
  55. Cox MJ, et al. 2021. Mol Ther. 29:1529. PubMed
  56. Cardoso V, et al. 2017. Nature. 549:277. PubMed
  57. Staessens S, et al. 2019. Haematologica. 104(10). PubMed
  58. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  59. Schwabenland M, et al. 2021. Immunity. . PubMed
  60. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  61. Rabilloud T, et al. 2021. Nat Commun. 12:865. PubMed
  62. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  63. Kanayama M, et al. 2021. Oncol Lett. 22:522. PubMed
  64. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  65. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  66. Hu Y, et al. 2016. J Exp Med. 213: 2759 - 2772. PubMed
  67. Jowett GM, et al. 2021. Nat Mater. 20:250. PubMed
  68. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  69. Crawford LB, et al. 2021. J Virol. 95:. PubMed
  70. Li H, et al. 2020. Haematologica. 105:1206. PubMed
  71. Jiang Q, et al. 2008. Blood. 112:2858. PubMed
  72. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  73. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  74. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
RRID
AB_314389 (BioLegend Cat. No. 304001)
AB_314389 (BioLegend Cat. No. 304002)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45 HI30 FC
Biotin anti-human CD45 HI30 FC
FITC anti-human CD45 HI30 FC
PE anti-human CD45 HI30 FC
PE/Cyanine5 anti-human CD45 HI30 FC
Purified anti-human CD45 HI30 FC,CyTOF®,IHC-P,WB,IHC-F,ICC
APC/Cyanine7 anti-human CD45 HI30 FC
PE/Cyanine7 anti-human CD45 HI30 FC
Alexa Fluor® 488 anti-human CD45 HI30 FC
Alexa Fluor® 647 anti-human CD45 HI30 FC
Pacific Blue™ anti-human CD45 HI30 FC
Alexa Fluor® 700 anti-human CD45 HI30 FC
PerCP anti-human CD45 HI30 FC
PerCP/Cyanine5.5 anti-human CD45 HI30 FC,SB
Brilliant Violet 421™ anti-human CD45 HI30 FC
Brilliant Violet 570™ anti-human CD45 HI30 FC
Brilliant Violet 510™ anti-human CD45 HI30 FC
Brilliant Violet 605™ anti-human CD45 HI30 FC
Brilliant Violet 650™ anti-human CD45 HI30 FC
Purified anti-human CD45 (Maxpar® Ready) HI30 FC,CyTOF®
Brilliant Violet 785™ anti-human CD45 HI30 FC
Brilliant Violet 711™ anti-human CD45 HI30 FC
PE/Dazzle™ 594 anti-human CD45 HI30 FC
Alexa Fluor® 594 anti-human CD45 HI30 IHC-P,SB
APC/Fire™ 750 anti-human CD45 HI30 FC
Pacific Blue™ anti-human CD45 HI30 FC
APC anti-human CD45 HI30 FC
PE/Cyanine7 anti-human CD45 HI30 FC
PE/Dazzle™ 594 anti-human CD45 HI30 FC
TotalSeq™-A0391 anti-human CD45 HI30 PG
TotalSeq™-B0391 anti-human CD45 HI30 PG
TotalSeq™-C0391 anti-human CD45 HI30 PG
PerCP/Cyanine5.5 anti-human CD45 HI30 FC
APC/Fire™ 750 anti-human CD45 HI30 FC
PE/Fire™ 640 anti-human CD45 HI30 FC
APC/Fire™ 810 anti-human CD45 HI30 FC
Spark YG™ 570 anti-human CD45 HI30 IHC-P
PE/Fire™ 700 anti-human CD45 HI30 FC
FITC anti-human CD45 HI30 FC
PerCP anti-human CD45 HI30 FC
Alexa Fluor® 660 anti-human CD45 Antibody HI30 FC
Spark Violet™ 538 anti-human CD45 HI30 FC
Spark YG™ 593 anti-human CD45 HI30 FC
GMP APC/Fire™ 750 anti-human CD45 HI30 FC
Spark Violet™ 538 anti-human CD45 HI30 FC
GMP APC anti-human CD45 HI30 FC
Spark UV™ 387 anti-human CD45 HI30 FC
GMP Pacific Blue™ anti-human CD45 HI30 FC
GMP PerCP anti-human CD45 HI30 FC
GMP FITC anti-human CD45 HI30 FC
PE anti-human CD45 HI30 FC
GMP PE/Dazzle™ 594 anti-human CD45 HI30 FC
GMP PerCP/Cyanine5.5 anti-human CD45 HI30 FC
Spark Blue™ 515 anti-human CD45 HI30 FC
TotalSeq™-D0391 anti-human CD45 HI30 PG
GMP PE/Cyanine7 anti-human CD45 HI30 FC
Spark Violet™ 500 anti-human CD45 HI30 FC
GMP PE anti-human CD45 HI30 FC
PE/Fire™ 810 anti-human CD45 HI30 FC
Spark PLUS UV395™ anti-human CD45 HI30 FC
Spark NIR™ 685 anti-human CD45 HI30 FC
Spark Violet™ 423 anti-human CD45 HI30 FC
Go To Top Version: 5    Revision Date: 08.09.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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