Purified anti-human CD50 ICAM-3 Antibody anti-CD50

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Clone: CBR-IC3/1 (See other available formats)
Regulatory Status: RUO
Other Names: ICAM-3
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes stained with purified CBR-IC3/1, followed by anti-mouse IgG FITC

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330002

100 µg

112 CHF

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Description

CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, α_L/β₂) integrin.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: SKW3 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: immunoprecipitation¹and blocking^1-3.
RRID: AB_1186142 (BioLegend Cat. No. 330002)

Antigen Details

Structure: Ig superfamily, type I membrane protein, 130 kD
Distribution: Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells
Function: Adhesion, costimulation
Ligand/Receptor: CD11a/CD18 (LFA-1), integrin α_L/ß₂
Cell Type: B cells, Endothelial cells, Granulocytes, Monocytes, T cells, Thymocytes
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Valent P, et al. 2001. Immunol. Rev. 179:74.
Gene ID: 3385 View all products for this Gene ID
UniProt: View information about CD50 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD50 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD50 (ICAM-3)	CBR-IC3/1	FC,IP
PE anti-human CD50 (ICAM-3)	CBR-IC3/1	FC
APC anti-human CD50 (ICAM-3)	CBR-IC3/1	FC
PerCP/Cyanine5.5 anti-human CD50 (ICAM-3)	CBR-IC3/1	FC
PE/Cyanine7 anti-human CD50 (ICAM-3)	CBR-IC3/1	FC

Certificate of Analysis

Go To Top Version: 3 Revision Date: 08.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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