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Purified anti-human CD52 Antibody

Pricing & Availability
Clone
HI186 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Cambridge pathology antigen 1 (CAMPATH-1), epididymal secretory protein 1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HI186_Purified_071307
Human peripheral blood lymphocytes stained with purified HI186, followed by anti-mouse IgGs FITC
  • HI186_Purified_071307
    Human peripheral blood lymphocytes stained with purified HI186, followed by anti-mouse IgGs FITC
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316002 100 µg 112 CHF
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Description

CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression. The HI186 antibody is useful for flow cytometry and immunohistochemistry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsil
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations
  1. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
RRID
AB_389275 (BioLegend Cat. No. 316002)

Antigen Details

Structure
Unusually small glycopeptide containing N-linked carbohydrate moiety, GPI-linked membrane protein, approximate molecular weight 7 kD
Distribution

Male reproductive tract (epididymis, seminal vesicle), T cells, B cells, macrophage/monocyte, eosinophil, erythrocytes, malignant lymphocytes

Function
Thought to play a role in carrying and orienting carbohydrates. Target for complement-mediated lysis and antibody-dependent cellular cytotoxicity, used as a depletion target for chronic lymphocytic leukemia/lymphoma therapy and immunosuppression.
Cell Type
B cells, Eosinophils, Erythrocytes, Macrophages, Monocytes, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997)
  2. Xia MQ, et al. 1991. Eur. J. Immunol. 21:1677.
  3. Kirchhoff C, et al. 1993. Mol. Reprod. Dev. 34:8.
  4. Xia MQ, et al. 1993. Biochem. J. 293:633.
Gene ID
1043 View all products for this Gene ID
UniProt
View information about CD52 on UniProt.org
Go To Top Version: 2    Revision Date: 08.27.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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