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Purified anti-human CD59 Antibody

Pricing & Availability
Clone
p282 (H19) (See other available formats)
Regulatory Status
RUO
Workshop
V S006
Other Names
Protectin, H19, 1F-5Ag, HRF20, MACIF, MIRL, P-18
Isotype
Mouse IgG2a, κ
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Product Citations
publications
p282_Purified_070207
Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC
  • p282_Purified_070207
    Human peripheral blood lymphocytes stained with purified p282, followed by anti-mouse IgGs FITC
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304701 25 µg 59 CHF
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304702 100 µg 114 CHF
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Description

CD59 is a 19-25 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as protectin, MACIF, and H19. It is broadly expressed on hematopoietic and non-hematopoietic cells. CD59 inhibits the cytolytic activity of complement by binding to C9, inhibiting incorporation into C5b-8, and preventing the terminal steps in complement polymerization of the membrane attack complex. CD59 has also been reported to play a role in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Yang J, et al. 2021. FEBS Lett. 595:1542. PubMed
RRID
AB_314453 (BioLegend Cat. No. 304701)
AB_314453 (BioLegend Cat. No. 304702)

Antigen Details

Structure
Ly6 superfamily, single chain GPI-linked membrane protein, 19-25 kD
Distribution

All cell types

Function
Blocks complement-mediated lysis by inhibiting MAC formation
Ligand/Receptor
Complement components C8, C9
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Davies A, et al. 1993. Immunol. Res. 12:258.
2. Lachmann P. 1991. Immunol. Today 12:312.
3. Liszewski M, et al. 1996. Adv. Immunol. 61:201.

Gene ID
966 View all products for this Gene ID
UniProt
View information about CD59 on UniProt.org
Go To Top Version: 2    Revision Date: 08.11.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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