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Purified anti-human CD66c/e Antibody

Pricing & Availability
Clone
3G5 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM 5/6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3G5_PURE_CD66c/e_Antibody_1_091521.png
Human lysed washed granulocytes stained with purified anti-human CD66c/e (clone 3G5) (filled histogram) or mouse IgG1, κ isotype control purified (open histogram) followed by anti-mouse IgG PE.
  • 3G5_PURE_CD66c/e_Antibody_1_091521.png
    Human lysed washed granulocytes stained with purified anti-human CD66c/e (clone 3G5) (filled histogram) or mouse IgG1, κ isotype control purified (open histogram) followed by anti-mouse IgG PE.
  • 3G5_PURE_CD66c/e_Antibody_2_091521.png
    Human colon cancer cell line HT-29 stained with purified anti-human CD66c/e (clone 3G5) (filled histogram) or mouse IgG1, κ isotype control purified (open histogram) followed by anti-mouse IgG PE.
  • 3G5_PURE_CD66c/e_Antibody_3_IHC_091521.png
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66c/e (clone 3G5) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • 3G5_PURE_CD66c/e_Antibody_4_IHC_091521.png
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD66c/e (clone 3G5) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
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364902 100 µg 147 CHF
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Description

CD66 plays a role in hemophilic and heterophilic adhesion. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904391 (BioLegend Cat. No. 364902)

Antigen Details

Structure
Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution

Granulocytes, epithelial cells

Function
Homophilic and heterophilic adhesion
Cell Type
Endothelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
Gene ID
1048 View all products for this Gene ID 4680 View all products for this Gene ID
UniProt
View information about CD66c/e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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