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Purified anti-human CD68 Recombinant Antibody

Pricing & Availability
Clone
QA20B07 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20B07_PURE_CD68_Antibody_032922
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of purified anti-human CD68 recombinant (clone QA20B07) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor™ 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor™ 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • QA20B07_PURE_CD68_Antibody_032922
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of purified anti-human CD68 recombinant (clone QA20B07) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor™ 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor™ 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
Cat # Size Price Quantity Check Availability Save
381202 100 µg 112 CHF
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Description

CD68, also known as macrosialin, is a 110 kD glycoprotein which belongs to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922613 (BioLegend Cat. No. 381202)

Antigen Details

Structure
Sialomucin family, 110 kD
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells

Molecular Family
CD Molecules
Antigen References
  1. Holness CL and Simmons DL. 1993. Blood. 81:1607-13.
  2. Gottfried E, et al. 2008. Scand J Immunol. 67:453-63.
  3. Hameed A, et al. 1994. Hum Pathol. 25:872-6.
Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.29.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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