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Purified anti-human CRP (C-reactive protein) Antibody

Pricing & Availability
Clone
A16065C (See other available formats)
Regulatory Status
RUO
Other Names
C-reactive protein (CRP), Pentraxin 1 (PTX1)
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_A16065C_PURE_CRP_Antibody_WB_041117
Total U87-MG cell lysates (negative control) and human serum protein (15µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-CRP antibody (clone A16065C) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Ponceau S staining was used as loading controls (lower). Lane M is the molecular weight ladder.
  • 1_A16065C_PURE_CRP_Antibody_WB_041117
    Total U87-MG cell lysates (negative control) and human serum protein (15µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL purified anti-CRP antibody (clone A16065C) antibody (upper). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Ponceau S staining was used as loading controls (lower). Lane M is the molecular weight ladder.
  • 2_A16065C_PURE_CRP_Antibody_IHC-P_072122
    IHC staining using purified anti-CRP (C-reactive protein) antibody (clone A16065C) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using 1X Tris-EDTA buffer (100 mM Tris, 10 mM EDTA, pH 9.0), the tissue was incubated with 10 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 µM.
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696006 100 µg 276 CHF
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Description

C-reactive protein (also known as CRP and Pentraxin 1 (PTX1)), is a member of pentraxin protein family. Similar to other pentraxins, CRP is a pattern recognition molecule that is able to recognize pathogens and promote their clearance. CRP tends to form a non-covalently linked pentamer with each subunit made up of two antiparallel β-sheets and a single short α-helix. It is characterized by the presence of a cleft that extends from the center of the subunit to its edge at the central pore of the pentamer structure. It has been demonstrated that CRP binds to the phosphocholine moieties expressed on the surface of dead or dying cells and some bacteria. This leads to the activation of the complement system and promotion of phagocytosis by macrophages to clear necrotic and apoptotic cells. CRP is secreted by hepatocytes and is a biomarker for inflammation. IL-6 has been shown to be an effective inducer of this protein. Besides binding to apoptotic cells, CRP also binds to a variety of molecules including C1q, bacterial polysaccharide, CD64, CD32, APCS, HDL, and fibronectin. In addition to participating in immune responses, CRP is also a prominent partaker in endothelial dysfunction and atherosclerosis. It was shown that CRP may exhibit direct proantherogenic effects by upregulating angiotensin type I receptor in smooth muscle cells and promoting their migration, proliferation, neointimal formation, and reactive oxygen species production. Mature human CRP shares 71% amino acid sequence homology with its mouse counterpart.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CRP/PTX1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunohistochemistry, a concentration range of 8 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For IHC-P, use 100 mM Tris, 10 mM EDTA, pH 9.0 for antigen retrieval buffer. Not recommended for use with Tris-buffered saline antigen retrieval buffer.

RRID
AB_2686984 (BioLegend Cat. No. 696006)

Antigen Details

Structure
Monomer and homopentamer
Distribution

Hepatocytes

Function
Induces expression of E-selectin and VCAM-1 expression in epithelial cells. CRP binds to target, initiates, and regulates complement activation. Induced by IL-1 and IL-6.
Interaction
Phosphocholine on the surface of apoptotic cells
Ligand/Receptor
Interacts with C1q, bacterial polysaccharide, CD64, CD32, APCS (PTX2), HDL, leptin, and fibronectin.
Biology Area
Cell Biology
Antigen References

1. Ansar W, Ghosh S. 2013. Immunol. Res. 56:131.
2. Agrawal A, et al. 2009. Adv. Exp. Med. Biol. 653:98.
3. Garlanda C, et al. 2005. Annu. Rev. Immunol. 23:337.
4. Verma S, et al. 2004. Circulation 109:1914.
5. Wadham C, et al. 2004. Circulation 109:2116.
6. Li XA, et al. 1998. Biochem. Biophys. Res. Commun. 244:249.
7. Lu J, et al. 2012. Immunol. Rev. 250:230.
8. Chen K, et al. 2006. Nat. Med. 12:425.

Gene ID
1401 View all products for this Gene ID
UniProt
View information about CRP on UniProt.org
Go To Top Version: 2    Revision Date: 07.21.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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