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Purified anti-human CX3CR1 Antibody

Pricing & Availability
Clone
K0124E1 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine C-X3-C receptor 1 (CX3CR1), V28, GPR13
Isotype
Mouse IgG1, κ
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Product Citations
publications
K0124E1_CX3CR1_Antibody_FC_1_112912
Human peripheral blood lymphocytes were stained with CD3 (clone HIT3a) Alexa Fluor® 647 and purified anti-human CX3CR1 (clone K0124E1, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • K0124E1_CX3CR1_Antibody_FC_1_112912
    Human peripheral blood lymphocytes were stained with CD3 (clone HIT3a) Alexa Fluor® 647 and purified anti-human CX3CR1 (clone K0124E1, top) or mouse IgG1, κ isotype control (bottom), followed by anti-mouse IgG1 PE.
  • K0124E1_CX3CR1_Antibody_FC_2_112912
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355701 25 µg 112 CHF
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355702 100 µg 229 CHF
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Description

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also known as GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known as fractalkine, neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediates cell firm adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DNA vaccine with the full coding sequence of human GPCR cloned in expression vector.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Breitmeyer R, et al. 2023. Commun Biol. 6:472. PubMed
  3. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  4. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  5. Schwabenland M, et al. 2021. Immunity. . PubMed
  6. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  7. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  8. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_2561725 (BioLegend Cat. No. 355701)
AB_2561725 (BioLegend Cat. No. 355702)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor
Distribution

NK cells, T cell subset, monocytes/macrophages, dendritic cells

Function
Cell adhesion and migration
Ligand/Receptor
CX3CL1 (fractalkine, FKN, neurotactin)
Cell Type
Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Imai T, et al. 1997. Cell. 91:521.
2. Fong AM, et al. 1998. J. Exp. Med. 188:1413.
3. Auffray C, et al. 2009. J. Exp. Med. 206:595.

Gene ID
1524 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 12.10.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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