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Purified anti-human FOXP3 Delta 2 (exon 2 deleted) Antibody

Pricing & Availability
Clone
A15080A (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A15080A_PURE_FOXP3_Antibody_013117
Total cell lysates (15 µg protein) from 293E untransfected (lane 1), 293E transfected with human FOXP3 (lane 2), and 293E transfected with human FOXP3 Delta2 (lane 3) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-FOXP3 Delta 2 (exon 2 deleted) antibody (clone A15080A). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • A15080A_PURE_FOXP3_Antibody_013117
    Total cell lysates (15 µg protein) from 293E untransfected (lane 1), 293E transfected with human FOXP3 (lane 2), and 293E transfected with human FOXP3 Delta2 (lane 3) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-FOXP3 Delta 2 (exon 2 deleted) antibody (clone A15080A). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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694802 100 µg 265 CHF
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Description

FOXP3 is a transcription factor which is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta 2) lacking exon 2. This antibody reacts to human FOXP3 delta 2 (exon 2 deleted) form and weakly reacted to the full-length FOXP3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human FOXP3 delta2 (exon 2 deleted) peptide conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is against human FOXP3 delta 2, molecular weight of 43 kD, and weakly reacts with full length human FOXP3, molecular weight 47 kD.

RRID
AB_2650734 (BioLegend Cat. No. 694802)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, exon 2 deleted, approximately 43 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells.

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis.
Interaction
IKZF3, STUB1, HSPA8 and HSPA1A/B, PPP1CA, PPP1CB, PPP1CG, KAT5, HDAC7, HDAC9, USP7, RUNX1, RORC, RUNX2, RUNX3 and IKZF4.
Cell Type
Tregs
Biology Area
Cell Biology, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References

1. Kristensen B, et al. 2015. Clin. Exp. Immunol. 180:58.
2. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.
3. Mayack S, et al. 2006. J. Immunol. 176:2059.
4. Yang ZZ, et al. 2006. Blood 107:3639.
5. Gavin MA, et al. 2006. Proc. Natl. Acad. Sci. USA 103:6659.
6. Groh V, et al. 2006. Nature Immunology 7:755.
7. Lewkowicz P, et al. 2006. J. Immunol. 177:7155.
8. Luke PP, et al. 2006. Am. J. Transplant. 6(9):2023.
9. Bamias G, et al. 2007. J. Immunol. 178:1809.
10. Valencia X, et al. 2007. J. Immunol. 178:2579.
11. Davidson TS, et al. 2007. J. Immunol. 178:4022. 

Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 1    Revision Date: 02.02.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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