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Purified anti-human HLA-G Recombinant Antibody

Pricing & Availability
Clone
QA19A11 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A11_PURE_HLA-G_Antibody_090122
Human cytotrophoblastic cell line (JEG-3) stained with purified anti-human HLA-G recombinant (clone QA19A11) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
  • QA19A11_PURE_HLA-G_Antibody_090122
    Human cytotrophoblastic cell line (JEG-3) stained with purified anti-human HLA-G recombinant (clone QA19A11) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
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381802 100 µg 110 CHF
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Description

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A, B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2 (CD85j), ILT4 (CD85d), and KIR2DL4 (CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in the thymus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA19A11 partially blocks the binding of reported clone MEMG/9.

RRID
AB_2924578 (BioLegend Cat. No. 381802)

Antigen Details

Structure
Ig
Distribution

Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors

Function
Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
Interaction
Dendritic cells, Epithelial cells, Macrophages, Monocytes
Ligand/Receptor
ILT2 (CD85j), ILT4 (CD85d), KIR2DL4 (CD158d), CD8α/α
Cell Type
Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References
  1. Hunt JS, et al. 2005. FASEB J. 19:681.
  2. Carosella ED, et al. 2008. Blood. 111:4862.
Gene ID
3135 View all products for this Gene ID
UniProt
View information about HLA-G on UniProt.org
Go To Top Version: 2    Revision Date: 08.28.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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