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Purified anti-human IGFALS Antibody

Pricing & Availability
Clone
M6005C04 (See other available formats)
Regulatory Status
RUO
Other Names
ACLSD, ALS, insulin-like growth factor binding protein, acid labile subunit
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M6005C04_Pure_IGFALS_Antibody_122618
  • M6005C04_Pure_IGFALS_Antibody_122618
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537302 500 µg 581 CHF
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Description

The insulin-like growth factors (IGFs) are found in binary complexes with members of IGF-binding protein family (IGFBP-1 to -6). IGFALS is the acid-labile subunit of the IGF binding protein complex that forms ternary complexes with IGFs and IGFBP-3/IGFBP-5. IGFALS has no affinity for free IGFs and has a very low affinity for uncomplexed IGFBP-3/IGFBP-5, but readily binds to the IGFs and IGFBP-3/IGFBP-5 complex, which extends the serum half-lives of IGFs. IGFALS is mainly produced by the liver and is exclusively found in high concentration in postnatal serum. IGFALS production is predominantly stimulated by the growth hormone. Mutations in IGFALS causes ALS, amyotrophic lateral sclerosis, deficiency, which manifests itself as delayed and slow puberty. IGFALS mutations have also been associated with insulin resistance. Mature IGFALS protein is composed of 578 amino acids with 20 leucine-rich repeats. It shares 93% identity between mouse and rat, and 79% between mouse and human.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1.0 - 8.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IGFALS recombinant protein ranging from 3.125 to 100 ng/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The pure M6005C04 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M6001E07 antibody (Cat. No. 537404) as the detection antibody and Recombinant Human IGFALS (ELISA Std.) (Cat. No. 758809).

Product Citations
  1. Kimura Y, et al. 2021. Sci Rep. 11:20638. PubMed
RRID
AB_2783348 (BioLegend Cat. No. 537302)

Antigen Details

Distribution

Produced by the liver and is mainly found in the postnatal serum.

Function
IGFALS production is stimulated by growth hormone and is important for extending the serum half life of IGF.
Interaction
IGF-1/IGF-2 and IGFBP-3/IGFBP-5 binary complexes
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Signal Transduction
Molecular Family
Carrier Proteins
Antigen References
  1. Baxter RC, Dai J. 1994. Endocrinology. 134:848-852.
  2. Twigg SM, Baxter RC. 1998. J Biol Chem. 273(11):6074-9.
  3. Khosravi MJ, et al. 1997. J Clin Endocrinol Metab. 82:3944-3951.
  4. Domene HM, et al. 2004. New Eng J Med. 350:570-577.
  5. Domene HM, et al. 2005. J Endocrinol Invest. 28(5):43-6.
Gene ID
3483 View all products for this Gene ID
UniProt
View information about IGFALS on UniProt.org
Go To Top Version: 1    Revision Date: 12.26.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-human IGFALS

    M6005C04_Pure_IGFALS_Antibody_122618
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