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Purified anti-human IL-12/IL-23 p40 (monomer, homodimer) Antibody

Pricing & Availability
Clone
C8.3 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Mouse IgG1, κ
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Product Citations
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Human_IL-12p40_020405
  • Human_IL-12p40_020405
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501702 500 µg 229 CHF
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Description

The C8.3 antibody reacts with human interleukin-12 and interleukin-23 (IL-12/IL-23) p40 monomer or homodimer. This antibody does not react with either the p35 subunit of IL-12 or the p19 subunit of IL-23, or the p40 protein when it is heterodimerized with p35 (the IL-12 p70) or p19 (IL-23 p60). The C8.3 antibody cannot neutralize the antagonistic activity of IL-12 p40.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
COS-expressed p40 subunit of human IL-12
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-12 (p40) recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture: The purified C8.3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C8.6 antibody (Cat. No. 508802/508805) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. This assay detects specifically human IL-12/IL-23 p40 protein, without interference by IL-12 p70 or IL-23 p19 proteins.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
  2. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
Product Citations
  1. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
  2. Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed
RRID
AB_2255186 (BioLegend Cat. No. 501702)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by hu
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells, PBL, mature dendritic cells
Receptors
IL-12Rβ1 binds p40 from both IL-12 and IL-23; dimeric with IL-12Rβ2 binds p35; IL-23 specific receptor subunit, IL-23R binds p19
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992. Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993. Immunol. Today. 14:335.
5. Oppmann B, et al. 2000. Immunity. 13:715.
6. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002. J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002. J. Immunol. 168:5448.
9. Lankford CS, et al. 2003. J. Leukoc. Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function
Gene ID
3593 View all products for this Gene ID
UniProt
View information about IL-12/IL-23 p40 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09.21.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-human IL-12/IL-23 p40 (monomer, homodimer)

    Human_IL-12p40_020405
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