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Purified anti-human IL-16 Antibody

Pricing & Availability
Clone
14.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-16
Isotype
Mouse IgG2a
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Product Citations
publications
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519102 100 µg 171 CHF
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Description

Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ELISA, IHC-F, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
  2. Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
  3. Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
  4. Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
  5. Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
Product Citations
  1. Colley T, et al. 2016. J Allergy Clin Immunol. 1595:137. PubMed
  2. Noman M, et al. 2015. Cancer Res . 75: 3771-3787. PubMed
RRID
AB_11204433 (BioLegend Cat. No. 519102)

Antigen Details

Structure
12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Distribution

Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.

Function
Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Ligand/Receptor
CD4.
Cell Type
Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.

Gene ID
3603 View all products for this Gene ID
UniProt
View information about IL-16 on UniProt.org
Go To Top Version: 4    Revision Date: 08.11.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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