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Purified anti-human IL-1α Antibody

Pricing & Availability
Clone
364-3B3-14 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)
Isotype
Mouse IgG1, κ
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Product Citations
publications
Poly5313_Biotin_IL-1a_Antibody
  • Poly5313_Biotin_IL-1a_Antibody
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500104 500 µg 270 CHF
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The 364-3B3-14 antibody reacts with human interleukin-1α (IL-1α). The 364-3B3-14 antibody is a non-neutralizing antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-1α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture1: The purified 364-3B3-14 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5313 antibody (Cat. No. 531304) as the detecting antibody and recombinant human IL-1a (Cat. No. 570009) as the standard. The Ultra-LEAF™ purified antibody (Cat. No. 500108) is suggested for ELISPOT capture.
Flow Cytometry4: The fluorochrome-labeled 364-3B3-14 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1a -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunohistochemistry3. The 364-3B3-14 is a non-neutralizing antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Thorpe R, et al. 1988. Lymphokine Res. 2:119.
  2. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
  3. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  4. Enriquez, J, et al. 2002. Adv. Perit Dial. 18:177.
  5. Lu Y, et al. 2015. PNAS. 112:607. PubMed
Product Citations
  1. Lu Y, et al. 2015. Proc Natl Acad Sci U S A. 112:607. PubMed
  2. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
RRID
AB_315083 (BioLegend Cat. No. 500104)

Antigen Details

Structure
Cytokine; 17.5 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cell Targets
B cells, T cells, monocytes, dendritic cells
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bomford, R., et al. Eds. 1989. Interleukin-1, inflammation and disease. Elsevier, New York.
3. Brazel, D., et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello, C. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
3552 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org

Other Formats

View All IL-1α Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human IL-1α 364-3B3-14 ICFC
Purified anti-human IL-1α 364-3B3-14 ELISA Capture,IHC
Ultra-LEAF™ Purified anti-human IL-1α 364-3B3-14 ELISA Capture,ELISPOT Capture,ICFC,IHC
TotalSeq™-B1203 anti-human IL-1α 364-3B3-14 ICPG
Go To Top Version: 5    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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