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Purified anti-human IRF3 Antibody

Pricing & Availability
Clone
AR1 (See other available formats)
Regulatory Status
RUO
Other Names
IRF3, IRF-3, interferon regulatory factor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AR1_PURE_IRF3_Antibody_dotplot_061419.png
Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat # 420801) and Permeablized with Intracellular Staining Permeabilization Wash Buffer (Cat #: 421002). Cells were then stained with anti-human IRF3 (clone AR1) Purified (left) or Mouse IgG1, κ Purified isotype control (right), followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE. Finally cells were washed and stained with anti-human CD3 (clone UCHT1) APC.
  • AR1_PURE_IRF3_Antibody_dotplot_061419.png
    Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat # 420801) and Permeablized with Intracellular Staining Permeabilization Wash Buffer (Cat #: 421002). Cells were then stained with anti-human IRF3 (clone AR1) Purified (left) or Mouse IgG1, κ Purified isotype control (right), followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE. Finally cells were washed and stained with anti-human CD3 (clone UCHT1) APC.
  • AR1_PURE_IRF3_Antibody_histogram_061419.png
    PC-3 (high IRF3 expression) cell line was fixed with Fixation Buffer (Cat # 420801) and permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat # 421002). Cells were then stained with anti-human IRF3 (clone AR1) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram), followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE.
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395902 100 µg 265 CHF
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Description

IRF-3 is a roughly 50 kD member of the interferon regulatory transcription factor (IRF) family. Along with other members of the IRF family IRF-3 plays a key role in the innate immune response to viral infection. It is located ubiquitously in an inactivated form in the cytoplasm of many immune cells. When phosphorylated it forms a complex with CREBBP to upregulate many immune responses integral to innate immune function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IRF-3 protein (entire protein)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Rustagi A, et al. 2012. Methods. 59(2):225-32
RRID
AB_2810597 (BioLegend Cat. No. 395902)

Antigen Details

Structure
Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD.
Distribution

Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.

Function
CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776
  2. Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654
  3. Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12
Gene ID
3661 View all products for this Gene ID
UniProt
View information about IRF3 on UniProt.org
Go To Top Version: 1    Revision Date: 06.14.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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