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Purified anti-human M-CSF Antibody

Pricing & Availability
Clone
L1617F1 (See other available formats)
Regulatory Status
RUO
Other Names
CSF1, CSF-1, MCSF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
L1617F1_Purified_M-CSF_Antibody_WB_043014.jpg
Western blot analysis of 50 ng of recombinant human M-CSF (lane 1) and 50 ng of recombinant mouse M-CSF (lane 2) using anti-human M-CSF antibody (clone L1617F1).
  • L1617F1_Purified_M-CSF_Antibody_WB_043014.jpg
    Western blot analysis of 50 ng of recombinant human M-CSF (lane 1) and 50 ng of recombinant mouse M-CSF (lane 2) using anti-human M-CSF antibody (clone L1617F1).
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526002 500 µg 347 CHF
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Description

M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNF-α, and IFN-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts and, consequently, this makes M-CSF a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF production in human monocytes and human tumor cells. High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human M-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2563444 (BioLegend Cat. No. 526002)

Antigen Details

Structure
Three isoforms with 256-554 amino acids, predicted molecular weight of approximately 30-70 kD.
Distribution

Cell membrane; single-pass membrane protein. Processed macrophage colony-stimulating factor 1: secreted, extracellular space.

Function
Controls the production, differentiation, and function of macrophages.
Interaction
Homodimer or heterodimer, disulfide-linked. Interacts with CSF1R.
Ligand/Receptor
M-CSFR or CSF1R (CD115).
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Kawasaki ES, et al. 1985. Science 230:291.
2. Wei S, et al. 2010. J. Leuko. Biol. 88:495.
3. Hodge JM, et al. 2011. PloS One 6:e21462.
4. Morandi A, et al. 2011. PloS One 6:e27450.
5. Erblich B, et al. 2011. PloS One 6:e26317.
6. MacDonald KP, et al. 2010. Blood 116:3955.

Gene ID
1435 View all products for this Gene ID
UniProt
View information about M-CSF on UniProt.org
Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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