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Purified anti-human MIF Antibody

Pricing & Availability
Clone
10C3 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF
Isotype
Mouse IgG2b, κ
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Product Citations
publications
MIF_Human_Antibody_ELISA_020514
  • MIF_Human_Antibody_ELISA_020514
  • n10C3_Purified_MIF_Antibody_WB_051314.jpg
    Western blot analysis of THP-1 (lane 1), Raw264.7(lane 2), PC-3(lane 3) and Jurkat(lane 4) cells using anti-MIF antibody (10C3). GAPDH antibody (poly6314) was used as loading control.
Cat # Size Price Quantity Check Availability Save
525501 50 µg 124 CHF
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525502 500 µg 358 CHF
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Description

Human macrophage migration inhibitory factor (MIF) is a 12.5 kD, 115 amino acid, non-glycosylated polypeptide expressed by multiple cell types, including activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. MIF plays many roles in biological processes such as catalytic activity, immunity, endocrine regulation, signal modulation, and inflammation.

MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors. Studies suggest that MIF might serve as a molecular link between chronic inflammation and cancer.

Recombinant MIF consists of a mixture of monomers, dimers, and trimers. The physiologically active forms are believed to be predominantly dimeric and trimeric forms.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MIF with a His tag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Verified
Recommended Usage

Each lot of this protein is quality control tested by ELISA assay.  A concentration of 4 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3 antibody (Cat. No. 525503) as the detection antibody.

Additional reported applications (for the relevant formats) include: functional assay1 and neutralization1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX™ ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhou H, et al. 2009. PLoS ONE. 4:e6087. (FA, Neut)
Product Citations
  1. Pérez-de Puig I, et al. 2013. J Cereb Blood Flow Metab. 1955:33. PubMed
RRID
AB_2563133 (BioLegend Cat. No. 525501)
AB_2563133 (BioLegend Cat. No. 525502)

Antigen Details

Structure
Predominantly a homotrimer
Distribution

Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells

Function
Pro-inflammatory cytokine, regulates macrophage function, involved in cancer development, tautomerase activity
Interaction
Monocytes and macrophages, mesenchymal cells, B-cells
Ligand/Receptor
CD74, CXCR4, CXCR2, CXCR7
Cell Type
Endothelial cells, Eosinophils, Epithelial cells, Macrophages, T cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Zhou H, et al. 2009. PLoS ONE. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K & Iczkowski K. 2005. BMC Cancer. 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.

Gene ID
4282 View all products for this Gene ID
UniProt
View information about MIF on UniProt.org

Other Formats

View All MIF Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human MIF 10C3 ELISA Capture,WB
Biotin anti-human MIF 10C3 ELISA Detection
Go To Top Version: 3    Revision Date: 05.13.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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