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Purified anti-human/mouse/rat PCNA Antibody

Pricing & Availability
Clone
PC10 (See other available formats)
Regulatory Status
RUO
Other Names
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Isotype
Mouse IgG2a, κ
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Product Citations
publications
a.PCNA
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • a.PCNA
    Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • PC10_Purified_PCNA_Antibody_2_010621
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-human/mouse/rat PCNA (clone PC10) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
  • PC10_Purified_PCNA_Antibody_3_031022_resized
    Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate Buffer 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 2.5 µg/ml of Purified anti-human/mouse/rat PCNA (clone PC10) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse secondary antibody (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective
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307901 25 µg 59 CHF
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307902 100 µg 114 CHF
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Description

The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat PCNA
Formulation
Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ICC, IHC-P - Verified
IP, WB, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.

Application References

(PubMed link indicates BioLegend citation)
  1. Ogata K, et al. 1985. J. Immunol. 135:2623.
  2. Garcia R, et al. 1989. Am. J. Pathol. 134:733.
  3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
  4. Waseem N, et al. 1990. J. Cell Sci. 96:121.
  5. Yu C, et al. 1991. Histopathology 19:29.
  6. Wilkins B, et al. 1992. J. Pathol. 166:45.
  7. Yang W, et al. 1996. Human Pathol. 27:70.
  8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
  10. Fulvio MD, et al. 2006. Oncogene 25:3932.
  11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
  12. Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  13. Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  14. Wallace HA, et al. 2014. Development. 141:1332. PubMed
  15. Mizokami A, et al. 2014. Bone. 69:68. PubMed
Product Citations
  1. Acevedo DS, et al. 2022. Neoplasia. 28:100791. PubMed
  2. Koliakou E, et al. 2022. Int J Mol Sci. 23: . PubMed
  3. Chen F, et al. 2023. Nat Commun. 14:336. PubMed
  4. Greenman R, et al. 2023. JCI Insight. 8:. PubMed
  5. Ho EK, et al. 2020. Current Biology. 30(14):2829-2835.e5. PubMed
  6. Eymann J, et al. 2020. Frontiers in Cell and Developmental Biology. 0.615277778. PubMed
  7. Mizokami A, et al. 2014. Bone. 69:68. PubMed
  8. Salomies L, et al. 2021. Sci Adv. 7:eabj7912. PubMed
  9. Moreno–Marín N, et al. 2018. iScience. 4:44. PubMed
  10. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  11. Brobeil A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
  12. Brummer G, et al. 2020. Oncogene. 39:2275. PubMed
  13. Salomies L et al. 2019. Elife. 8 pii: e47702. PubMed
  14. Wallace H, et al. 2014. Development. 141:1332. PubMed
  15. Gal O, et al. 2022. J Nanotheranostics. 3:177. PubMed
  16. Schlessinger V 2007. Proc Natl Acad Sci U S A. 104:3937. PubMed
  17. Eymann J et al. 2019. The Journal of comparative neurology. 527(14):2356-2370 . PubMed
  18. Moreno-Marín N, et al. 2017. Sci Rep. 10.1038/s41598-017-10984-w. PubMed
  19. Hu Q, et al. 2019. Biol Open. 8. PubMed
  20. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  21. Padavannil A, et al. 2019. Elife. 8. PubMed
  22. Fang WB, et al. 2021. Sci Rep. 11:8708. PubMed
  23. Tsai HH, et al. 2020. Sci Rep. 0.640277778. PubMed
  24. Wang JT et al. 2017. eLife. 6 pii: e29061. PubMed
  25. Henkels K, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
  26. Lan T, et al. 2015. PLoS One. 10: 0129743. PubMed
  27. Perini S, et al. 2016. Rev Port Pneumol (2006). 22:209-213. PubMed
  28. Wang Q, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_314691 (BioLegend Cat. No. 307901)
AB_314691 (BioLegend Cat. No. 307902)

Antigen Details

Structure
DNA-polymerase sliding clamp, trimeric ring; 36 kD
Distribution

Nuclear, all proliferating cells

Function
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Interaction
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Ligand/Receptor
Ubiquitination, Sumoylation
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Antigen References

1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.

Gene ID
5111 View all products for this Gene ID
UniProt
View information about PCNA on UniProt.org

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Go To Top Version: 6    Revision Date: 08.11.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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