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Purified anti-human Notch 2 Antibody

Pricing & Availability
Clone
MHN2-25 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 2, hN2, fN2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
MHN2-25_PE_data1_040810
Human Notch2 transfected CHO cells stained with purified MHN2-25 conjugated to PE
  • MHN2-25_PE_data1_040810
    Human Notch2 transfected CHO cells stained with purified MHN2-25 conjugated to PE
  • MHN2-25_PE_data2_040810
    Human pre-B cell line NALM16 stained with MHN2-25 PE
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348301 25 µg 124 CHF
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Description

The Notch receptors are a type I transmembrane protein family that share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. They are highly conserved from invertebrates to mammals. Notch 1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors (Notch1-4).  Notch1 and Notch 2 are reported to be functionally redundant.  Notch 2 is expressed by Pre-B cells, double negative and CD8 single positive thymocytes, brain, lung cells. Notch 2 functions as a receptor for Jagged and Delta ligands, and may play a role in vascular, renal and hepatic development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Notch 2-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Haraguchi K, et al. 2009. J. Immunol. 182:6168.
RRID
AB_10551623 (BioLegend Cat. No. 348301)

Antigen Details

Distribution

Double negative and CD8 single positive thymocytes, pre-B cells, brain, lung

Ligand/Receptor
Jag 1, Jag 2; Delta 1, Delta 4
Cell Type
B cells, Neural Stem Cells, Thymocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Haraguchi K, et al. 2009. J. Immunol. 182:6168.
2. O’Neill CF, et al.2007. Am. J. Path. 171:1023.
3. Bertrand FE, et al. 2000. Leukemia 14:2095.

Gene ID
4853 View all products for this Gene ID
UniProt
View information about Notch 2 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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