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Purified anti-human Notch 3 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
MHN3-21 (See other available formats)
Regulatory Status
RUO
Other Names
Neurogenic locus notch homolog protein 3, Notch-3, CADASIL
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHN3-21_Purified_Notch3_Cytof_061014.jpg
Human Jurkat T cells (top) and human NALM-6 pre-B cells (bottom) stained with 165Ho-anti-Notch 3 (MHN3-21). Data provided by DVS Sciences.
  • MHN3-21_Purified_Notch3_Cytof_061014.jpg
    Human Jurkat T cells (top) and human NALM-6 pre-B cells (bottom) stained with 165Ho-anti-Notch 3 (MHN3-21). Data provided by DVS Sciences.
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345407 100 µg 258 CHF
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Description

The Notch receptors are highly conserved from invertebrates to mammals. While Notch 1 and Notch 2 exhibit the highest structural similarity among the four mammalian Notch receptors. Notch 3 has a number of structural and functional differences. The binding of Notch 3 to its ligands results in the proteolysis of Notch and movement of intracellular portions of Notch into the nucleus. This translocation triggers a series of signaling processes. Notch 3 is primarily expressed in adult arterial smooth muscle cells. Notch3 gene mutation can cause CADASIL, a hereditary early stroke syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Notch 3-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

RRID
AB_2563786 (BioLegend Cat. No. 345407)

Antigen Details

Structure
Transmembrane receptor, heterodimer consisting of a C-terminal fragment and N-terminal fragment probably linked by disulphide bonds. Contains 5 ankyrin repeats, 29-36 EGF repeats, 3 Notch/Lin repeats.
Cell Type
Tregs
Biology Area
Immunology
Antigen References

1. Ehebauer ME. et al. 2006. Biochem J 392:13
2. Joutel A et al. 1996. Nature 383:707
3. Shimizu K et al. 2000. Mol Cell Biology 20:18
4. Tanigaki K et al. 2007. Nature Immunol 8:451
5. Bellavia D et al. 2008. 27:5092
6. Louvi A et al. Dev Neurosci. 2006. 28:5

Gene ID
4854 View all products for this Gene ID
UniProt
View information about Notch 3 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 2    Revision Date: 07.22.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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