- Clone
- mAb 84 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1, GCTM2
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![mAb_84_PURE_Podocalyxin_Antibody_090117 mAb_84_PURE_Podocalyxin_Antibody_090117](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/mAb_84_PURE_Podocalyxin_Antibody_081817.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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iPS cells (NC6) were stained with purified PODXL (clone mAb 84, filled histogram) or mouse IgM, κ isotype control (open hisotgram), followed by anti-mouse IgM PE. -
Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and purified PODXL (clone mAb 84, left) or mouse IgM, κ isotype control (right), followed by anti-mouse IgM PE. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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374302 | 100 µg | 270 CHF |
Podocalyxin-like protein-1 (also known as PODXL, GP200, PCLP1 and GCTM2) is expressed on the surface of undifferentiated human ES cells and glomerular podocytes. PODXL was originally described as the main sialoprotein expressed by glomerular epithelial cells. Recently PODXL been associated with several forms of cancer and is being targeted as a possible therapeutic. Clone mAb 84 is reported to recognize and have cytotoxic affects against undifferentiated human ES cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Cyt, ICC, IP, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for relevant formats) include: WB, IP, cytotoxicity. The LEAF purified format is recommended for cytotoxicity assays.
- RRID
-
AB_2715813 (BioLegend Cat. No. 374302)
Antigen Details
- Structure
- Sialomucin family
- Distribution
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Stem cells, Glomerular podocytes
- Function
- Stem cell differentiation, regulatory factor
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
- Antigen References
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1. Kerjaschki D, et al. 1984. J Cell Bio. 98:1591.
2. Horvat R, et al. 1986. J Cell Bio. 102:484.
3. Choo AB, et al. 2008. Stem Cells. 26:1454.
4. Tan HL, et al. 2009. Stem Cells. 27:1792.
5. Nielsen JS, et al. 2009. J Am Soc Nephrol. 20:1669. - Gene ID
- 5420 View all products for this Gene ID
- UniProt
- View information about Podocalyxin on UniProt.org
Related FAQs
Other Formats
View All Podocalyxin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Podocalyxin | mAb 84 | FC,Cyt,ICC,IP,WB |
FITC anti-human Podocalyxin | mAb 84 | FC |
TotalSeq™-C1051 anti-human Podocalyxin | mAb 84 | PG |
TotalSeq™-B1051 anti-human Podocalyxin Antibody | mAb 84 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Podocalyxin
iPS cells (NC6) were stained with purified PODXL (clone mAb ... Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 ... -
FITC anti-human Podocalyxin
Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 ... -
TotalSeq™-C1051 anti-human Podocalyxin
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TotalSeq™-B1051 anti-human Podocalyxin Antibody
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