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Purified anti-human Sialyl Lewis X (dimeric) Antibody

Pricing & Availability
Clone
FH6 (See other available formats)
Regulatory Status
RUO
Other Names
CD15s, Stage-Specific Embryonic Antigen 1, SSEA-1, Sialyl Lewis X, sLex, sLe(x), Sialyl demeric LeX, Sialyl dermic Lewis X, Sialyl Lewis X-i, sLeX-i
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
FH6_PURE_SialylLewisX_Antibody_1_FC_081915
Human peripheral blood granulocytes were stained with purified sialyl lewis X (dimeric) (clone FH6) (filled histogram) or mouse IgM, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • FH6_PURE_SialylLewisX_Antibody_1_FC_081915
    Human peripheral blood granulocytes were stained with purified sialyl lewis X (dimeric) (clone FH6) (filled histogram) or mouse IgM, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • FH6_PURE_SialylLewisX_Antibody_2_FC_081915
    Human peripheral blood lymphocytes were stained with purified sialyl lewis X (dimeric) (clone FH6) (filled histogram) or mouse IgM, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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368102 100 µg 229 CHF
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Description

The FH6 antibody recognizes Sialyl Lewis X (demeric) on glycolipids or glycoproteins. It also recognizes Sialyl Lewis X with long carbohydrate attachments (Sialyl Lewis X-i). These antigens are expressed on human granulocytes, monocytes, small subsets of lymphocytes, some fetal tissues such as the fetal stomach, fetal colon, and fetal intestine, and a variety of cancer tissues. It is believed that these antigens are involved in cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified 6B fucoganglioside absorbed to Salmonella minnesota.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC, RIA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
  2. Fukushi Y, et al. 1985. Cancer Res. 8:3711.
  3. Kannagi R, et al. 1986. Cancer Research 5:2619.
  4. Kobayashi M, et al. 2010. Anticancer Res. 30:593.
RRID
AB_2566069 (BioLegend Cat. No. 368102)

Antigen Details

Structure
Carbohydrate epitope; VI3NeuAcV3III3Fuc2nLc6.
Distribution

Granulocytes, monocytes, subpopulations of lymphocytes, fetal stomach, fetal colon, fetal small intestine, and a variety of cancer tissues.

Function
Cell adhesion.
Ligand/Receptor
Selectins.
Cell Type
Granulocytes, Monocytes, Lymphocytes, Embryonic Stem Cells, Neutrophils, Neural Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Fukushi Y, et al. 1984. J. Biol. Chem. 259:10511.
2. Kannagi R, et al. 1986. Cancer Research 5:2619.
3. Nakasaki H, et al. 1989. Cancer Research 49:3662.
4. Dohi T, et al. 1993. Anticancer Res. 13:1277.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about Sialyl Lewis X on UniProt.org
Go To Top Version: 1    Revision Date: 08.21.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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