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Purified anti-human STAT3 Antibody

Pricing & Availability
Clone
15H2B45 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, acute-phase response factor (APRF), HIES, ADMIO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
15H2B45_PURE_STAT3_Antibody_082216
HeLa cells (filled histogram) or PC-3 cells (open histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with purified STAT3 (clone 15H2B45), followed by anti-mouse IgG PE.
  • 15H2B45_PURE_STAT3_Antibody_082216
    HeLa cells (filled histogram) or PC-3 cells (open histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with purified STAT3 (clone 15H2B45), followed by anti-mouse IgG PE.
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371802 100 µg 265 CHF
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Description

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 15H2B45 blocks clone 4G4B45.

RRID
AB_2629732 (BioLegend Cat. No. 371802)

Antigen Details

Structure
770 amino acids with a predicted molecular weight of 88.1 kD. Contains a C-terminal SH2 domain responsible for homo- or heterodimerization.
Distribution

Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to nucleus to act as transcriptional activator. Activates a wide variety of genes involved in cell growth and apoptosis, involved in gp130-related signaling.

Function
Cytoplasm.
Interaction
STAT3 forms a homodimer or a heterodimer with other STAT family members. Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lufei C, et al. 2003. EMBO J. 22:1325.
2. Deo D, et al. 2002. J. Biol. Chem. 277:21237.
3. Pfeffer L, et al. 1997. Science. 276:1418.
4. Akira S, et al. 1994. Cell. 77:63.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.23.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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