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Purified anti-human VEGFR1 Antibody

Pricing & Availability
Clone
A16083C (See other available formats)
Regulatory Status
RUO
Other Names
Fms-related tyrosine kinase 1, oncogene FLT, FLT, FLT-1, VEGFR1, VEGFR-1, VEGFR
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16083C_Pure_VEGFR1_Antibody_031721
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human VEGFR1 (clone A16083C) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A16083C_Pure_VEGFR1_Antibody_031721
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human VEGFR1 (clone A16083C) at 4°C overnight, followed by 2.5 µg/mL of anti-mouse IgG (clone Poly4053) Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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394902 100 µg 229 CHF
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Description

VEGF-R1, also known as Fms-related tyrosine kinase 1 (FLT-1), is type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases.  Human VEGF-R1 is mainly expressed by endothelial cells, which plays central role in vasculogenesis, angiogenesis and lymphaniogenesis. VEGFR-1 is the only VEGFR present on the surface of monocytes, and it mediates a chemotactic response to VEGF-A and tissue factor induction. It also reported on placental trophoblast cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells.  VEGFR1  is regulated by PEDF, which effects its intracellular proteolysis rate. sVEGFR1 regulates the availability of VEGFA and is produced in the placenta under hypoxic conditions. The ligands of VEGF-R1 include VEGF-A, VEGF-B, and PIGF splice isoforms.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human VEGFR1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890833 (BioLegend Cat. No. 394902)

Antigen Details

Structure
Cytokine receptor
Distribution

Endothelial cells, placental trophoblast cells, peripheral blood monocytes and macrophages

Function
Angiognesis and vasculogenesis
Ligand/Receptor
VEGFR-A, VEGFR-B, and PlGF
Antigen References

1. Kendall RL, et al. 1996. Biochem. Biophys. Res. Commun. 226:324.
2. Cai J, et al. 2006. J. Biol. Chem. 281:3604.
3. Ambati BK, et al. 2006. Nature 443:993.
4. Heydarian M, et al. 2009. Placenta 30:250.
5. Thomas CP, et al. 2009. J. Clin. Endocrinol. Metab. 94:2524. 
6. Wu, et al. 2010. J. Cell Mol. Med. 14:528.
7. Cai J, et al. 2011. PLoS One 6:e18076.

Gene ID
2321 View all products for this Gene ID
UniProt
View information about VEGFR1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02.18.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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