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Purified anti-IDH1 Antibody

Pricing & Availability
Clone
O92H9 (See other available formats)
Regulatory Status
RUO
Other Names
Isocitrate Dehydrogenase 1 (NADP+), Soluble, Oxalosuccinate Decarboxylase, NADP(+)-Specific ICDH, PICD, IDH, IDP, Isocitrate Dehydrogenase [NADP] Cytoplasmic, Cytosolic NADP-Isocitrate Dehydrogenase, IDCD
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_O92H9_Pure_IDH1_Antibody_1_WB_110117
Total lysates (10µg protein) from HeLa and HeLa IDH1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-IDH1 antibody , clone O92H9 (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-IDH1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder.
  • 1_O92H9_Pure_IDH1_Antibody_1_WB_110117
    Total lysates (10µg protein) from HeLa and HeLa IDH1 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-IDH1 antibody , clone O92H9 (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-IDH1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder.
  • 2_O92H9_Pure_IDH1_Antibody_1_WB_072116
    Total cell lysate from NIH3T3 cells (lane 1, 15 µg) and HeLa cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-IDH1 antibody (Clone O92H9) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-β-actin antibody (poly6221) was used as a loading control.
  • 3_O92H9_PURE_IDH1_Antibody_IHC_010518
    HeLa cells were fixed with ice cold methanol for five minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/mL anti-human IDH1 (clone O92H9) for two hours followed by 2 µg/mL Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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685302 100 µg 265 CHF
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Description

IDH1, also known as Isocitrate Dehydrogenase 1, enzyme plays and important role in lipid biosynthesis in different tissues such as the liver, adipose tissues, brain and tumors. IDH1 normally catalyze the decarboxylation of isocitrate to generate α-ketoglutarate (αKG), but recurrent mutations at Arg132 of IDH1 confer a neomorphic enzyme activity that catalyzes reduction of αKG into the putative oncometabolite D-2-hydroxyglutate (D2HG). D2HG inhibits αKG-dependent dioxygenases and is thought to create a cellular state permissive to malignant transformation by altering cellular epigenetics and blocking normal differentiation processes. To date, mutations in the IDH1 gene have been found in numerous cancers with the highest frequencies occurring in gliomas, chondrosarcomas or enchondromas, and cholangiocarcinomas. In addition, mutation of IDH1 is closely related to the progress and prognosis of certain tumors. Therefore, IDH1 is considered to be a promising biomarker for early diagnosis, prognosis and targeted therapy for tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified full length recombinant IDH1 expressed in 293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Does not react with mouse IDH1.

For immunofluorescence microscopy, positive staining was observed in the cytoplasm of HeLa cells.

RRID
AB_2616768 (BioLegend Cat. No. 685302)

Antigen Details

Structure
414 amino acids with the predicted molecular weight of approximately 47 kD.
Distribution

Cytoplasm and peroxisome.

Function
IDH1 enzyme plays an important role in lipid biosynthesis in different tissues such as the liver, adipose tissues, brain and tumors. Mutation of IDH is closely related to the progress and prognosis of certain tumors.
Interaction
Isocitrate
Biology Area
Cancer Biomarkers, Cell Biology
Antigen References

1. De Quintana-Schmidt C, et al. 2015. Neurocirugia (Astur.) 26:276.
2. Waitkus MS, et al. 2016. Neuro. Oncol. 18:16.
3. Liu X, et al. 2015. Histol. Histopathol. 30:1155.
4. Fathi AT, et al. 2015. Semin. Hematol. 52:165.
5. Vigneswaran K, et al. 2015. Ann. Transl. Med. 3:95.
6. Bogdanovic E. 2015. Biochim. Biophys. Acta. 1850(9):1781-5.
7. Parker SJ, et al. 2015. Pharmacol. Ther. 152:54.
8. Krell D, et al. 2013. Future Oncol. 9:1923.
9. McKenney AS, et al. 2013. J. Clin. Invest. 123:3672.

Gene ID
3417 View all products for this Gene ID
UniProt
View information about IDH1 on UniProt.org

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Go To Top Version: 3    Revision Date: 11.01.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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