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Purified anti-IGF1 Antibody

Pricing & Availability
Clone
1500102 (See other available formats)
Regulatory Status
RUO
Other Names
Insulin-Like Growth Factor 1, IGF-1, Mechano Growth Factor, Somatomedin-C, IGF-I, MGF, Insulin-Like Growth Factor IA, Insulin-Like Growth Factor IB, Nonsuppressible insulin-like activity I (NSILA-I)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1500102_PURE_IGF1_Antibody_WB_092915
Recombinant human IGF-I (50ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IGF1 (clone 1500102) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • 1500102_PURE_IGF1_Antibody_WB_092915
    Recombinant human IGF-I (50ng) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IGF1 (clone 1500102) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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680402 100 µg 276 CHF
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Description

IGF-I, also known as insulin-like growth factor 1), was initially described in 1957 as a hormone that is important for child development, and also has anabolic effects in adults. It is a member of the IGF family that is comprised of three members: IGF-I, IGF-II, and insulin. IGF-I and IGF-II share approximately 50% similarity with insulin at the amino acid level. Human IGF-I is synthesized as a 191 amino acid prepro-IGF-I; pro-IGF-I is processed to the mature, active protein, and this procedure requires glucose regulating protein 94 (GRP94) that possesses chaperone activity towards IGF-I. Deletion of muscle GRP94 impairs muscle and body growth by inhibiting local production of IGF proteins. Seven IGF binding proteins have been described (IGFBP-1 to -7). IGFBPs extend the half-lives and regulate the availability of IGF-I and IGF-II. It has been described that IGFBP3 is the main circulating binding protein for IGFs in serum. 75% of circulating IGF-I and IGF-II bind to IGFBP3 and the co-carrier acid labile subunit (ALS). IGF-I binds to three receptors, and the binding to IGFIR promotes cell proliferation, survival, differentiation, and migration. IGF-I deficiency is associated with growth hormone insensitivity syndrome (GHIS), which is also called Laron syndrome, liver cirrhosis, age-related cardiovascular, neurological diseases, and intrauterine growth restriction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human IGF-I, amino acids (Gly49-Ala118) (Accession# PO05019), expressed in E. coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566356 (BioLegend Cat. No. 680402)

Antigen Details

Structure
The 70 amino acid recombinant protein has a predicted molecular weight of approximately 7.6 kD. The predicted N terminal amino acid is Gly.
Distribution
Secreted protein.
Function
Similar to insulin in function and structure and is involved in mediating growth and development.
Interaction
IGF binding protein 1, IGF binding protein 2, IGF binding protein 3, IGF binding protein 4, IGF binding protein 5, IGF binding protein 6, and Fibronectin 1.
Ligand/Receptor
IGF1R.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Bell GI, et al. 1984. Nature 310:775.
2. Karey KP, Sirbasku DA. 1988. Cancer Res. 48:4083.
3. Mohan S, Baylink DJ. 2002. J. Endocrinol. 1:19.
4. Ostrovsky O, et al. 2010. Biochim. Biophys. Acta. 1803:333.
5. Barton ER, et al. 2012. FASEB. J. 26:3691.
6. Puche JE, Castilla-Cortazar I. 2012. J. Transl. Med. 10:224.
7. Ni F, et al. 2013. Nat. Commun. 4:1479.

Gene ID
3479 View all products for this Gene ID
UniProt
View information about IGF1 on UniProt.org
Go To Top Version: 1    Revision Date: 09.29.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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