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Purified anti-IL-12/IL-23 p40 Antibody

Pricing & Availability
Clone
A15076D (See other available formats)
Regulatory Status
RUO
Other Names
IL-12 subunit p40, IL-23 subunit p40, Cytotoxic lymphocyte maturation factor, CLMF2, Natural killer cell stimulatory factor 40-KD subunit, NKSF2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A15076D_PURE_IL-12_IL-23_p40_Antibody_101716
THP-1 cells were non-treated or treated with 100 ng/ml PMA for 3 days, and then treated with 1 µg/ml LPS for 1 day. Total protein extracts (15 µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-IL-12/IL-23 p40 (clone A15076D) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
  • A15076D_PURE_IL-12_IL-23_p40_Antibody_101716
    THP-1 cells were non-treated or treated with 100 ng/ml PMA for 3 days, and then treated with 1 µg/ml LPS for 1 day. Total protein extracts (15 µg protein) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-IL-12/IL-23 p40 (clone A15076D) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
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692502 100 µg 253 CHF
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Description

IL-12 and IL-23 share the p40 subunit, which heterodimerizes respectively with IL-12 p35 or IL-23 p19 subunits to form IL-12 or IL-23. IL-12 p40 exists as a monomer and as a homodimer (IL-12 p80). IL-12 acts as a growth factor for activated human T and NK cells, enhances the lytic activity of human NK cells, and stimulates the production of IFN-γ by resting human PBMC. IL-12R is formed by two chains, IL-12Rβ1 and IL-12Rβ2. IL-12Rβ1 is associated with the Janus kinase (Jak) Tyk2 and binds IL-12 p40; IL-12Rβ2 is associated with Jak2 and binds either the heterodimer or the p35 chain. Signaling through the IL-12 receptor complex induces phosphorylation, dimerization, and nuclear translocation of several signal transducers and activators of transcription (STAT) family members (STAT1, 3, 4, 5), but most of the biological responses to IL-12 have been attributed to STAT4. IL-12 has been shown to elicit anti-tumor activity in mice and humans. It is believed that the antitumor effects of IL-12 are mediated, at least in part, by indirect mechanisms. Induction of IFN-γ results in the upregulation of class I and class II MHC molecules, adhesion molecules (ICAM-1), nitric oxide production by antigen presenting cells (APC), and the production of additional cytokines, CXCL9 and 10, which in turn mediate angiostatic effects.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-12.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632765 (BioLegend Cat. No. 692502)

Antigen Details

Structure
328 amino acids with a predicted molecular weight of 40 kD.
Distribution

Secreted.

Function
p40 homodimer functions as a proinflammatory protein that enhances leukocyte accumulation in the skin, blunt Th1 immunity to Plasmodium berghei, and provides protective immunity toward mycobacterial infection. In addition, p40 homodimer induces macrophage chemotaxis independent of IL-12. Also, IL-12 p40 homodimer (p402) induces the expression of inducible nitric oxide synthase (iNOS) in microglia.
Interaction
Heterodimer with IL12A and IL23A.
Ligand/Receptor
IL-12Rβ1.
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schoenhaut DS, et al. 1992. J. Immunol. 148:3433.
2. Manetti R, et al. 1994. J. Exp. Med. 179:1273.
3. Ireland D, et al. 2005. Viral Immunol. 18:397.
4. Moreno SE, et al. 2006. J. Immunol. 177:3218.
5. Lyakh L, et al. 2008. Immunol. Rev. 226:112.
6. Theiner G, et al. 2008. Mol. Immunol. 45:244.
7. Zhu S, et al. 2010. J. Immunol. 184:2348.

Gene ID
3593 View all products for this Gene ID
UniProt
View information about IL-12/IL-23 p40 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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