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Purified anti-IL-27 p28 Antibody

Pricing & Availability
Clone
B0315A10 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-27 Subunit Alpha, IL-27 (28K subunit)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
B0315A10_Purified_IL-27_p28_Antibody_WB_050115
Western blot analysis of 50 ng recombinant human IL-27 (lane 1) and recombinant mouse IL-27 (lane 2) using anti-IL-27 P28 antibody (clone B0315A10) and HRP anti-mouse IgG antibody.
  • B0315A10_Purified_IL-27_p28_Antibody_WB_050115
    Western blot analysis of 50 ng recombinant human IL-27 (lane 1) and recombinant mouse IL-27 (lane 2) using anti-IL-27 P28 antibody (clone B0315A10) and HRP anti-mouse IgG antibody.
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676902 100 µg 229 CHF
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Description

IL-27 is a heterodimeric cytokine that is composed of two subunits (p28 and EBI3). It plays a critical role in T-cell proliferation and differentiation. It also drives rapid clonal expansion of naive but not memory CD4 T cells. IL-27 is a potent inhibitor of Th17 cell development and IL-17 production. IL-27 subunit p28 alone is able to inhibit the production of IL-17 by CD4 and CD8 T cells. Furthermore, IL-27 plays an important role in antitumor and antiangiogenic activity through the increase in the production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-27.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565723 (BioLegend Cat. No. 676902)

Antigen Details

Structure
A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40 related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution

Monocytes, macrophages, and dendritic cells (DCs).

Function
T cell proliferation and differentiation.
Interaction
Activated T cells, resting natural killer cells (NK), resting NKT cells, neutrophils, eosinophils, mast cells, hepatic stellate cells, hepatoma cells, and hepatocytes.
Ligand/Receptor
IL-27RA, also known as WSX-1 or TCCR, paired with gp130.
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pflanz S, et al. 2002. Immunity 16:779.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Batten M, et al. 2006. Nat. Immunol. 7:929.
4. Villarino AV, et al. 2006. J. Immunol. 176:237.
5. Stumhofer JS, et al. 2007. Nat. Immunol. 8:1363.
6. Awasthi A, et al. 2007. Nat. Immunol. 8:1380.
7. Pot C, et al. 2009. J. Immunol. 183:797.
8. Bender H, et al. 2009. Hepatology 50:585.
9. Bosmann M and Ward PA. 2013. J. Leukoc. Biol. 94:1159.

Gene ID
246778 View all products for this Gene ID
UniProt
View information about IL-27 on UniProt.org
Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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