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Purified anti-IL-34 Antibody

Pricing & Availability
Clone
C051-77 (See other available formats)
Regulatory Status
RUO
Other Names
IL34, Interleukin 34, Chromosome 16 Open Reading Frame 77, C16orf77
Isotype
Rat IgG2c, κ
Ave. Rating
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Product Citations
publications
1_C051-77_Purified_IL-34_Antibody_031519_updated.png
Total cell lysates (15 µg total protein) from Human and mouse brain tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-IL34 antibody, clone C051-77, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_C051-77_Purified_IL-34_Antibody_031519_updated.png
    Total cell lysates (15 µg total protein) from Human and mouse brain tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-IL34 antibody, clone C051-77, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_C051-77_IL-34_Antibody_IP_022715.jpg
    Immunoprecipitation/Western blot analysis using the anti-IL-34 antibody (C051-77). 100 ng/mL of recombinant mouse IL-34 was tested. Lane 1 was immunoprecipitated with rat IgG control antibody and lane 2 was immunoprecipitated with anti-IL-34 antibody (C051-77). Western blot analysis was performed using goat anti-mouse IL-34 antibody.
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675102 100 µg 265 CHF
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Description

IL-34 is a glycoprotein that plays important roles in the proliferation, survival, and differentiation of monocytes and macrophages. IL-34 is expressed in the heart, brain, lung, liver, kidney, thymus, spleen, and other organs. It specifically binds to CD115/M-CSF receptor (CSF-1 receptor or c-Fms) and activates ERK1/2 phosphorylation. The function of IL-34 overlaps with CSF-1 to regulate myeloid lineage development. IL-34 also promotes inflammatory processes and regulates osteoclast proliferation, differentiation, and bone reabsorption.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse IL-34 produced in CHO cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2 - 5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The theoretical MW of IL-34 is 27 kD, the observed MW is 50 kD.

RRID
AB_2565492 (BioLegend Cat. No. 675102)

Antigen Details

Structure
215 amino acids with a predicted molecular weight of approximately 26 kD.
Distribution

Expressed in different tissues including the spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostrate, and colon.

Function
Regulates myeloid lineage differentiation, proliferation, and survival.
Interaction
Macrophages, monocytes, and myeloid cells.
Ligand/Receptor
CD115/M-CSF receptor (CSF-1 receptor or c-Fms)
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lin H, et al. 2008. Sciences 320:807.
2. Chihara T, et al. 2010. Cell Death Differ. 17:1917.
3. Wei S, et al. 2010. J. Leuko. Biol. 88:495.
4. Eda H, et al. 2011. Rheumatol. 31:1525.
5. Covaleda L, et al. 2010. Virology 397:217.

Gene ID
762577 View all products for this Gene ID
UniProt
View information about IL-34 on UniProt.org
Go To Top Version: 3    Revision Date: 06.07.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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