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Purified anti-IMPDH1 Antibody

Pricing & Availability
Clone
1D6-E3 (See other available formats)
Regulatory Status
RUO
Other Names
Inosine Monophosphate Dehydrogenase 1, Inosine-5'-Monophosphate Dehydrogenase 1, IMP Dehydrogenase 1, IMPD1, IMP (Inosine Monophosphate) Dehydrogenase 1, Inosine Monophosphate Dehydrogenase Type 1, IMPDH 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1D6-E3_PURE_IMPDH1_Antibody_1_022520
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-IMPDH1 antibody (clone 1D6-E3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • 1D6-E3_PURE_IMPDH1_Antibody_1_022520
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-IMPDH1 antibody (clone 1D6-E3) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • 1D6-E3_PURE_IMPDH1_Antibody_2_022520
    Whole cell extracts (250 µg total protein) prepared from MOLT4 cells were immunoprecipitated overnight with 2.5 µg of purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 401402) or purified anti-IMPDH1 antibody (clone 1D6-E3). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of IMPDH. Lane M: Molecular weight marker.
  • 1D6-E3_PURE_IMPDH1_Antibody_3_021820
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1 µg/mL of either purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 401402) (panel A) or purified anti-IMPDH1 antibody (clone 1D6-E3) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60x objective.
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935201 25 µg 101 CHF
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935202 100 µg 253 CHF
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Description

Inosine-5'-monophosphate dehydrogenase 1 (IMPDH1) was initially identified as an oncogene in several human cancers. IMPDH1 catalyzes the conversion of inosine-5'-monophosphate (IMP) to xanthosine-5'-monophosphate (XMP) and is the first committed rate-limiting step in the de novo synthesis of GTP, and consequently it is involved in the regulation of cell growth. IMPDH1 is also part of the composition of the cytoplasmic rod and ring structures (RR) and is involved in maintaining cellular nucleotide pools needed for DNA and RNA synthesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length IMPDH purified from a Chinese hamster V79 cell variant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes human, mouse and hamster IMPDH1 and is predicted to recognize the rodent orthologs. Clone is likely to cross react with IMPDH2.

For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 µg/mL.

RRID
AB_2832890 (BioLegend Cat. No. 935201)
AB_2832890 (BioLegend Cat. No. 935202)

Antigen Details

Structure
IMPDH1 is an 514 aminoacid protein with a predicted molecular weight of 56 kD.
Distribution

Localized to the cytosol filamentous rods and rings / Ubiquitously expressed

Function
Regulation of cell growth, and biosynthesis of de novo GTP
Biology Area
Cell Cycle/DNA Replication, Cell Proliferation and Viability, DNA Repair/Replication
Antigen References
  1. Calise SJ, et al. 2015. Front Immunol. 6:41.
  2. Liao LX, et al. 2017. Proc Natl Acad Sci U S A. 29.
  3. Duan S, et al. 2018. J Exp Clin Cancer Res. 37:304.
  4. Zhao Y, et al. 2018. Biomarkers. 1:9.
  5. Carcamo WC, et al. 2011. PLoS One. 6.
  6. Xu Y, et al. 2017. Sci Rep. 7:745.
  7. Glesne DA, et al. 1991. Mol Cell Biol. 11:5417-25.
  8. Hager PW, et al. 1995. Biochem Pharmacol. 49:1323-9.
Gene ID
3614 View all products for this Gene ID
UniProt
View information about IMPDH1 on UniProt.org
Go To Top Version: 2    Revision Date: 10.28.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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