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Purified anti-Insulin Degrading Enzyme (IDE) Antibody

Pricing & Availability
Clone
9B12.225 (See other available formats)
Regulatory Status
RUO
Other Names
Abeta-degrading protease, Insulin protease, Insulinase, Abeta-Degrading Protease
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9B12dot225_Insulin_Degrading_Enzyme_Anitbody_1_021218
Western blot of anti-Insulin Degrading Enzyme (IDE) antibody (clone 9B12.225 ). Lane 1: Molecular weight marker; Lane 2: 30 µg of human colon lysate; Lane 3: 30 µg of human skeletal muscle lysate. The blot was incubated with 5 µg/ml of primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9B12dot225_Insulin_Degrading_Enzyme_Anitbody_1_021218
    Western blot of anti-Insulin Degrading Enzyme (IDE) antibody (clone 9B12.225 ). Lane 1: Molecular weight marker; Lane 2: 30 µg of human colon lysate; Lane 3: 30 µg of human skeletal muscle lysate. The blot was incubated with 5 µg/ml of primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9B12dot225_Insulin_Degrading_Enzyme_Anitbody_2_021218
    Western blot of anti-Insulin Degrading Enzyme (IDE) antibody (clone 9B12.225 ). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/ml of primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse secondary antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 9B12dot225_Insulin_Degrading_Enzyme_Anitbody_3_060118
    Total lysates (15 µg protein) from HeLa (Human), CHO (Hamster) and UMR106 (Rat) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/ml purified anti-Insulin Degrading Enzyme (IDE) antibody, clone 9B12.225. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
Cat # Size Price Quantity Check Availability Save
921203 25 µg 118 CHF
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921204 100 µg 293 CHF
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Description

Insulin degrading enzyme (IDE), also known as insulinase, insulin protease or insulysin, cleaves the peptide hormone insulin with consequences for insulin response and resistance. Since its discovery, IDE has been shown to degrade a variety of bioactive peptides. Recently the finding that IDE clears intracellular and extracellular amyloid products has spurred research into the link between IDE function and human neurodegenerative disease such as Alzheimer’s.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against purified human erythrocyte IDE
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 9B12.225 has been shown to detect a ~118 kD isoform in peripheral tissue and a second isoform at ~54 kD in brain tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. RG Bennett, et al. 2000. Endocrinology. 141:2508. (WB)
  2. Chou YH, et al. 2009. FASEB J. 23:3734. (ICC) PubMed
RRID
AB_2728611 (BioLegend Cat. No. 921203)
AB_2728611 (BioLegend Cat. No. 921204)

Antigen Details

Structure
IDE is a 1019 amino acid protein with a molecular weight of ~ 118 kD.
Distribution

Tissue distribution: Abundantly expressed in gastrointestinal tract, muscle and nervous system tissues.
Cellular distribution: Secreted, mitochondrion, peroxisome, nucleus, cytosol, and plasm membrane.

Function
IDE cleaves the peptide hormone insulin with consequences for insulin response and resistance. IDE is also the enzyme responsible for degradation and clearance of amyloid beta in the brain.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Enzymes and Regulators, Proteases
Antigen References
  1. Tang WJ. 2016. Trends Endocrinol Metab. 27(1):24.
  2. Jha NK, et al. 2015. J Alzheimers Dis. 48(4):891.
Gene ID
3416 View all products for this Gene ID
UniProt
View information about Insulin Degrading Enzyme on UniProt.org
Go To Top Version: 2    Revision Date: 06.01.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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