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Purified anti-IRF1 Antibody

Pricing & Availability
Clone
13H3A44 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 1 (IRF-1)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
13H3A44_PURE_IRF1_Antibody_1_WB_062116
Total lysates (15 µg protein) from HeLa (lane 1), Molt-4 (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-IRF1 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 13H3A44_PURE_IRF1_Antibody_1_WB_062116
    Total lysates (15 µg protein) from HeLa (lane 1), Molt-4 (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-IRF1 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • 13H3A44_PURE_IRF1_Antibody_2_IF_121817
    HeLa cells were fixed with 2% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-IRF1 Antibody (clone 13H3A44) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 13H3A44_PURE_IRF1_Antibody_3_IP_121817
    Immunoprecipitation of IRF1 from Hela cells treated with human IFNg (100 ng/ml, 4 hr). Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-IRF1 antibody (clone 13H3A44, lane 4). Western blot was performed using anti-IRF1 antibody (clone 13H3A44).
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657602 100 µg 265 CHF
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Description

IRF1 is the first identified member of the interferon regulatory transcription factor family. Its expression is induced in response to pathogen infection or the stimulation of several cytokines, including type I and II IFN, IL-1, IL-6, and TNF-α. Upon stimulation, IRF1 is transcriptionally activated by STAT1 and NFκB transcription factors via the GAS (interferon-gamma-activated sequence) element in the IRF1 promoter. IRF1 plays important  roles in mediating immune response during infection and development of T cells and NK cells. In addition to the immunomodulatory functions, IRF1 has also shown to be involved in the regulation of cell cycle progression, tumor suppression, and apoptosis. IRF1-mediated transcription can be repressed by IRF2 through competing for the same binding region on the promoters.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF1 recombinant protein (147-325 a.a.)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted at 4°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Velásquez LN, et al. 2017. J Leukoc Biol. 101:759. PubMed
  2. Bailey KA, et al. 2019. FASEB J. 33:12888. PubMed
RRID
AB_2562631 (BioLegend Cat. No. 657602)

Antigen Details

Structure
325 amino acids, predicted Mw is 37 kD and observed MW is 45kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.
Distribution

Nucleus.

Function
IRF1 is a transcription factor, regulating expression of IFN and IFN-inducible genes. It is also involved in regulating cell proliferation, apoptosis, tumor suppression, and various immune responses.
Interaction
Forms homodimers. Interacts with p300, MyD88, and PIAS3.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Transcription Factors
Antigen References

1. Yang W, et al. 2012. PLoS One 7:e50932.
2. Schwartz JL, et al. 2011. Int. J. Breast Cancer. 2011:912102.
3. Horiuchi M, et al. 2011. J. Neuroinflammation. 8:8.
4. Frontini M, et al. 2009. Nucleic Acids Res. 37:1073.
5. Yarilina A, et al. 2008. Nat. Immunol. 9:378.
6. Kano S, et al. 2008. Nat. Immunol. 9:34.

Gene ID
3659 View all products for this Gene ID
UniProt
View information about IRF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 9    Revision Date: 01.13.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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