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Purified anti-IRF7 Antibody

Pricing & Availability
Clone
12G9A36 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor-7 (IRF-7)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
a12G9A36_Purified_IRF7_Antibody_WB_062713
Total lysates (15 µg protein) from Jurkat (lane 1) and Jurkat treated with 100 ng/mL recombinant human IFN-α cells for 16h (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:2500 diluted (0.2 µg/mL) purified anti-IRF7 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • a12G9A36_Purified_IRF7_Antibody_WB_062713
    Total lysates (15 µg protein) from Jurkat (lane 1) and Jurkat treated with 100 ng/mL recombinant human IFN-α cells for 16h (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:2500 diluted (0.2 µg/mL) purified anti-IRF7 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • b12G9A36_Purified_IRF7_Antibody_IF_062713
    Total cell lysates from untreated Jurkat cells (Lane 1) or Jurkat cells treated with IFNα (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IRF7 (clone 12G9A36) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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656002 100 µg 347 CHF
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Description

IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial IRF7 recombinant protein (127-503 a.a.)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2562245 (BioLegend Cat. No. 656002)

Antigen Details

Structure
503 amino acids, predicted molecular weight of 54 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
Distribution

Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus

Function
Transcription factor that transactivates proinflamatory cytokines and type I interferon genes in response to virus infection
Interaction
Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References

1. Owens BM, et al. 2012. PLoS One. 7:e41050.
2. Yu Y, et al. 2010. Immunity. 33:863.
3. Liang Q, et al. 2011. J. Immunol. 186:1001.
4. Buss C, et al. 2010. J. Immunol. 184:3072.
5. Joo CH, et al. 2007. J. Virol. 81:8282.
6. Balachandran S, et al. 2007. J. Immunol. 178:2429.

Gene ID
3665 View all products for this Gene ID
UniProt
View information about IRF7 on UniProt.org
Go To Top Version: 3    Revision Date: 01.12.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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